Homer B L, Pierce K R
Department of Veterinary Pathology, College of Veterinary Medicine, Texas A & M University, College Station 77843-4463.
Histochem J. 1989 Feb;21(2):63-71. doi: 10.1007/BF01005981.
The density of hepatocellular catalase-containing peroxisomes was quantified, utilizing a computer-aided image analysing technique, on 1-micron thick diaminobenzidine-stained sections. Hepatic copper accumulation following intraperitoneal injection of cupric chloride resulted in a dose-dependent reduction in the density of catalase-containing peroxisomes. A significant correlation between the density of peroxisomes and the activity of hepatic catalase indicated that computer-aided image analysis of peroxisomes stained by the diaminobenzidine technique provided a useful estimate of catalase activity in liver injured by copper. Slight treatment-related differences in the mean diameter of peroxisomes were detected in high-dose but not low-dose rats.
利用计算机辅助图像分析技术，对1微米厚的二氨基联苯胺染色切片上含过氧化氢酶的肝细胞过氧化物酶体密度进行了定量分析。腹腔注射氯化铜后肝脏铜蓄积导致含过氧化氢酶的过氧化物酶体密度呈剂量依赖性降低。过氧化物酶体密度与肝脏过氧化氢酶活性之间存在显著相关性，这表明通过二氨基联苯胺技术染色的过氧化物酶体的计算机辅助图像分析为铜损伤肝脏中过氧化氢酶活性提供了有用的估计。在高剂量而非低剂量大鼠中检测到过氧化物酶体平均直径存在与处理相关的细微差异。
