De Craemer D, Pauwels M, Van den Branden C
Menselijke Anatomie and Embryologie, Vrije Universiteit Brussel, Belgium.
Hepatology. 1995 Sep;22(3):744-52. doi: 10.1002/hep.1840220309.
We investigated the hepatocellular peroxisomes in 27 patients with steatosis of the liver by means of catalase cytochemistry, light and electron microscopic study, and morphometry. Seven normal human livers were used as controls. In our patients, fatty liver was mainly associated with alcohol abuse or obesity. Indications for a slight decrease in catalase activity and for a proliferation were found in visual evaluation of the peroxisomes. Morphometric analysis showed a significant decrease in mean peroxisomal diameter (to 87%) and a simultaneous significant elevation to numerical density of the peroxisomes (to 188%); this resulted in a normal volume density and a significant increase to (133%) in surface density. However, individual differences were found. No differences in peroxisomal characteristics were found between fatty livers of different causes. A significant inverse linear correlation between mean peroxisomal diameter and numerical density was found in patients with fatty livers. Because a similar correlation was also found when control data were added to the fatty liver data, we hypothesize that the peroxisomal compartment in human fatty livers is adapted in such a way to permit the same metabolic efficiency as in control livers.
我们通过过氧化氢酶细胞化学、光镜和电镜研究以及形态测量法，对27例肝脂肪变性患者的肝细胞过氧化物酶体进行了研究。选取7例正常人类肝脏作为对照。在我们的患者中，脂肪肝主要与酒精滥用或肥胖有关。在对过氧化物酶体的视觉评估中，发现过氧化氢酶活性略有下降和过氧化物酶体增殖的迹象。形态测量分析显示，过氧化物酶体平均直径显著减小（降至87%），而过氧化物酶体的数量密度同时显著升高（升至188%）；这导致了正常的体积密度，表面密度显著增加（升至133%）。然而，发现了个体差异。不同病因的脂肪肝之间过氧化物酶体特征未发现差异。在脂肪肝患者中，过氧化物酶体平均直径与数量密度之间存在显著的负线性相关性。由于将对照数据添加到脂肪肝数据中时也发现了类似的相关性，我们推测人类脂肪肝中的过氧化物酶体区室以这种方式进行了适应性调整，以实现与对照肝脏相同的代谢效率。
