Guangorena-Gómez J O, Maravilla-Domínguez A, García-Arenas G, Cervantes-Flores M, Meza-Velázquez R, Rivera-Guillén M, Acosta-Saavedra L C, Goytia-Acevedo R C
Facultad de Medicina, Universidad Juárez del Estado de Durango (UJED), Gómez Palacio, Durango, México.
Facultad de Ciencias Químicas, UJED, Durango, Durango, México.
Parasite Immunol. 2016 Aug;38(8):468-80. doi: 10.1111/pim.12334. Epub 2016 Jun 8.
It has been demonstrated that the allergic response can be ameliorated by the administration of pathogen derivatives that activate Toll-like receptors and induce a Th1-type immune response (IR). Cryptosporidium is a parasite that promotes an IR via Toll-like receptors and elicits the production of Th1-type cytokines, which limit cryptosporidiosis. The aim of this study was to investigate allergy-related immune markers in children naturally infected with Cryptosporidium. In a cross-sectional study, 49 children with or without clinical diagnosis of allergies, oocysts of Cryptosporidium spp. in the faeces were screened microscopically. We microscopically screened for leucocytes, examined T and B cells for allergy-related activation markers using flow cytometry and evaluated serum for total IgE using chemiluminescence. Children with allergies and Cryptosporidium in the faeces had significantly lower levels of total IgE, B cells, CD19(+) CD23(+) and CD19(+) CD124(+) cells as well as a greater percentage of interferon-gamma (IFN-γ(+) ) and IL-4(+) CD4(+) cells than children with allergies without Cryptosporidium. This is the first description of the modulation of the IR in children with allergic diseases in the setting of natural Cryptosporidium infection. Our findings suggest the involvement of CD4(+) cells producing IL-4 and IFN-γ in the IR to Cryptosporidium in naturally infected children.
已经证明，通过给予激活Toll样受体并诱导Th1型免疫反应（IR）的病原体衍生物，可以改善过敏反应。隐孢子虫是一种通过Toll样受体促进免疫反应并引发Th1型细胞因子产生的寄生虫，这些细胞因子可限制隐孢子虫病。本研究的目的是调查自然感染隐孢子虫的儿童中与过敏相关的免疫标志物。在一项横断面研究中，对49名有或无过敏临床诊断的儿童进行粪便中隐孢子虫属卵囊的显微镜筛查。我们通过显微镜筛查白细胞，使用流式细胞术检测T细胞和B细胞的过敏相关激活标志物，并使用化学发光法评估血清中的总IgE。粪便中有过敏和隐孢子虫的儿童与无隐孢子虫的过敏儿童相比，总IgE、B细胞、CD19(+) CD23(+)和CD19(+) CD124(+)细胞水平显著降低，而干扰素-γ（IFN-γ(+)）和IL-4(+) CD4(+)细胞的百分比更高。这是首次描述自然感染隐孢子虫情况下过敏性疾病儿童免疫反应的调节。我们的研究结果表明，在自然感染儿童中，产生IL-4和IFN-γ的CD4(+)细胞参与了对隐孢子虫的免疫反应。
