Jannesar Ramin, Zare Fahimeh, Ghaedi Mehrorang, Daneshfar Ali
Department of Pathology, Yasuj University of Medical Sciences, Yasuj, Iran.
Department of Chemistry, Faculty of Sciences, Yasouj University, Yasouj 75918-74831, Iran.
Ultrason Sonochem. 2016 Sep;32:380-386. doi: 10.1016/j.ultsonch.2016.04.010. Epub 2016 Apr 7.
A two-step sample preparation technique based on dispersive micro solid-phase extraction combined with coacervative microextraction is presented for preconcentration and determination of tricyclic antidepressant drugs in biological samples. An important feature of the method is the application of hydrophobic magnetic nanoparticles, which in combination with coacervative microextraction method enables development of rapid and efficient extraction procedure in order to achievement of a high extraction efficiency. Simultaneous optimization by experimental design lead to improvement of method with low cost which supply useful information about interaction among variables. Under the optimized conditions, a linear range of 5-1000ngmL(-1) with detection limits from 0.51 to 1.4ngmL(-1) were obtained for target analytes. The method was successfully used for the determination of analytes in biological fluids (plasma and urine) with relative recoveries in the range of 89-105% (RSDs<3.5%).
本文提出了一种基于分散微固相萃取结合凝聚微萃取的两步样品制备技术，用于生物样品中三环类抗抑郁药物的预富集和测定。该方法的一个重要特点是应用了疏水性磁性纳米颗粒，其与凝聚微萃取方法相结合，能够开发出快速高效的萃取程序，以实现高萃取效率。通过实验设计进行同步优化，以低成本改进了方法，提供了有关变量间相互作用的有用信息。在优化条件下，目标分析物的线性范围为5-1000ngmL(-1)，检测限为0.51至1.4ngmL(-1)。该方法成功用于生物流体（血浆和尿液）中分析物的测定，相对回收率在89-105%范围内（相对标准偏差<3.5%）。
