D'souza Brendan, Nayak Ramakant, Kotrashetti Vijayalakshmi S
Department of Oral Pathology and Microbiology, Maratha Mandal NG Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
Appl Immunohistochem Mol Morphol. 2018 Feb;26(2):132-139. doi: 10.1097/PAI.0000000000000383.
Oral leukoplakia is the most common premalignant lesion of the oral cavity and is associated with development of oral squamous cell carcinoma. Certain changes at cellular and molecular level are important indicators for transformation into carcinoma. Podoplanin, a transmembrane glycoprotein is involved in the cytoskeletal remodeling and increased motility of the cell which helps in determining the malignant potential of oral leukoplakic lesions. The objective of the study was to determine immunohistochemically the expression of podoplanin in homogenous (HOL) and nonhomogenous oral leukoplakia (NHOL) and to compare the expression with clinicopathological parameters. Study group included 15 cases each of HOL, NHOL and control group included 15 healthy volunteers. Both tissues were immunohistochemically stained for podoplanin (D2-40) antibody. No statistical significant difference was observed between the study and control groups for expression of podoplanin but significant difference was observed on comparison of podoplanin scores between HOL and NHOL. Statistical significant difference was observed when the podoplanin expression in the epithelium and the lymphatic vessel density were correlated with the histologic grading of HOL and NHOL. Expression of podoplanin was greater in NHOL as compared with HOL, this supports the fact that NHOL has a greater risk of malignant transformation when compared with HOL. Podoplanin expression, lymphangiogenesis, and lymphatic vessel density increased with increasing grades of dysplasia, suggesting that cellular modeling and motility is increased as the grade of dysplasia advances. Thus suggesting podoplanin can be used as a prognostic marker to determine the malignant potential in oral leukoplakias.
口腔白斑是口腔最常见的癌前病变，与口腔鳞状细胞癌的发生有关。细胞和分子水平的某些变化是向癌转化的重要指标。血小板内皮细胞黏附分子（Podoplanin）是一种跨膜糖蛋白，参与细胞骨架重塑和细胞运动性增加，这有助于确定口腔白斑病变的恶性潜能。本研究的目的是通过免疫组织化学方法确定血小板内皮细胞黏附分子在均质型口腔白斑（HOL）和非均质型口腔白斑（NHOL）中的表达，并将其表达与临床病理参数进行比较。研究组包括15例HOL和15例NHOL，对照组包括15名健康志愿者。两组组织均用血小板内皮细胞黏附分子（D2-40）抗体进行免疫组织化学染色。研究组和对照组在血小板内皮细胞黏附分子表达上未观察到统计学显著差异，但在比较HOL和NHOL的血小板内皮细胞黏附分子评分时观察到显著差异。当血小板内皮细胞黏附分子在上皮中的表达和淋巴管密度与HOL和NHOL的组织学分级相关时，观察到统计学显著差异。与HOL相比，NHOL中血小板内皮细胞黏附分子的表达更高，这支持了NHOL与HOL相比具有更高恶性转化风险这一事实。随着发育异常程度的增加，血小板内皮细胞黏附分子表达、淋巴管生成和淋巴管密度增加，这表明随着发育异常程度的进展，细胞形态和运动性增加。因此表明血小板内皮细胞黏附分子可作为一种预后标志物来确定口腔白斑的恶性潜能。
