与百草枯反应的单克隆抗体的研制与特性鉴定

Development and characterization of monoclonal antibodies reactive with paraquat.

作者信息

Nagao M, Takatori T, Wu B, Terazawa K, Gotouda H, Akabane H

机构信息

Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.

出版信息

J Immunoassay. 1989;10(1):1-17. doi: 10.1080/01971528908053224.

DOI:10.1080/01971528908053224

PMID:2715345

Abstract

A set of three anti-paraquat monoclonal antibodies(MoAbs), named APM-1, APM-2 and APM-3, has been isolated. In order to evaluate the ability of these MoAbs to recognize various kinds of bipyridyl herbicides and similar congeners of paraquat, a competition enzyme-linked immunosorbent assay (ELISA) using avidin-biotin complex (ABC) was developed. All three antibodies strongly recognized paraquat and slightly did the other analogs. These three MoAbs are therefore advantageous to a toxicological study of paraquat and of its localization in tissues.

摘要

已分离出一组三种抗百草枯单克隆抗体（MoAbs），命名为APM-1、APM-2和APM-3。为了评估这些单克隆抗体识别各种联吡啶类除草剂和百草枯类似同系物的能力，开发了一种使用抗生物素蛋白-生物素复合物（ABC）的竞争酶联免疫吸附测定（ELISA）。所有三种抗体都能强烈识别百草枯，对其他类似物的识别较弱。因此，这三种单克隆抗体有利于百草枯的毒理学研究及其在组织中的定位研究。