Johnston S C, Bowles M, Winzor D J, Pond S M
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Fundam Appl Toxicol. 1988 Aug;11(2):261-7. doi: 10.1016/0272-0590(88)90150-9.
The techniques of in vitro and in vivo immunization have been used to produce murine hybridomas secreting anti-paraquat IgM and IgG antibodies, respectively. The monoclonal antibodies from these IgM-secreting and IgG-secreting cell lines have been purified by HPLC and characterized by ELISA for their affinities to eliciting antigen and cross-reactivity. Both antibody preparations exhibited high selectivity and similar affinities for paraquat. There was only a minor degree of interaction with a range of paraquat analogs that differed in the nature and position of group substituents.
