Baines M G, Rafferty B, Patel V, Ferguson K, Jeffcoate S L, Thorpe R
Division of Immunobiology and Endocrinology, National Institute for Biological Standards and Control, Herts, UK.
J Immunoassay. 1989;10(1):75-91. doi: 10.1080/01971528908053228.
Monoclonal antibodies against human prolactin (PRL) have been produced and characterised and used to develop a sensitive two-site immunoradiometric assay (IRMA). Nine anti-PRL monoclonal antibodies were assessed for reactivity in immunoblotting experiments with PRL, hPL, hGH and pituitary gland extract. There was no detectable crossreactivity with hPL or hGH. In liquid phase radioimmunoassay (RIA) studies using three of the antibodies there was no detectable crossreaction from hPL or hGH. Five antibodies were positive in immunocytochemical studies using sections of human pituitary gland. Using FPLC purified monoclonal antibodies, a two-site IRMA was developed that could assay PRL over the range 17.5-3500 mIU per litre and was readily adapted to assaying serum samples from patients. The two-site IRMA could be performed within one day without loss of sensitivity and has potential as a rapid and simple method for screening clinical samples.
已制备并鉴定了抗人催乳素（PRL）的单克隆抗体，并用于开发一种灵敏的双位点免疫放射分析（IRMA）。评估了九种抗PRL单克隆抗体在与PRL、人胎盘催乳素（hPL）、人生长激素（hGH）和垂体提取物的免疫印迹实验中的反应性。未检测到与hPL或hGH的交叉反应。在使用其中三种抗体的液相放射免疫分析（RIA）研究中，未检测到hPL或hGH的交叉反应。在使用人垂体切片的免疫细胞化学研究中，五种抗体呈阳性。使用快速蛋白质液相色谱（FPLC）纯化的单克隆抗体，开发了一种双位点IRMA，其可测定每升17.5 - 3500 mIU范围内的PRL，并且易于用于检测患者的血清样本。该双位点IRMA可在一天内完成，且不会损失灵敏度，具有作为筛查临床样本的快速简便方法的潜力。
