Mave V, Chandanwale A, Kinikar A, Khadse S, Kagal A, Gupte N, Suryavanshi N, Nimkar S, Koli H, Khwaja S, Bharadwaj R, Joshi S, Horng H, Benet L Z, Ramachandran G, Dooley K E, Gupta A, Gandhi M
Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India; Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Byramjee-Jeejeebhoy Medical College Clinical Trials Unit, Pune, India.
Int J Tuberc Lung Dis. 2016 Jun;20(6):844-7. doi: 10.5588/ijtld.15.0882.
Assessing treatment adherence and quantifying exposure to anti-tuberculosis drugs among children is challenging. We undertook a 'proof of concept' study to assess the drug concentrations of isoniazid (INH) in hair as a therapeutic drug monitoring tool. Children aged <12 years initiated on a thrice-weekly treatment regimen including INH (10 mg/kg) for newly diagnosed tuberculosis were enrolled. INH concentrations in hair were measured using liquid chromatography-tandem mass spectrometry at 1, 2, 4 and 6 months after initiating anti-tuberculosis treatment. We found that INH hair concentrations in all children on thrice-weekly INH were detectable and displayed variability across a dynamic range.
评估儿童的治疗依从性以及量化其抗结核药物暴露情况具有挑战性。我们开展了一项“概念验证”研究,以评估头发中异烟肼(INH)的药物浓度作为一种治疗药物监测工具。纳入了年龄小于12岁、开始接受包括INH(10mg/kg)在内的每周三次治疗方案用于新诊断结核病的儿童。在开始抗结核治疗后的1、2、4和6个月,使用液相色谱-串联质谱法测量头发中的INH浓度。我们发现,所有接受每周三次INH治疗的儿童头发中的INH浓度均可检测到,并且在动态范围内呈现出变异性。