Wigley K, Wakelin S A, Moot D J, Hammond S, Ridgway H J
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, New Zealand.
AgResearch Ltd, Christchurch, New Zealand.
J Appl Microbiol. 2016 Aug;121(2):495-505. doi: 10.1111/jam.13170. Epub 2016 Jun 30.
The aim of this work was to develop a tool to investigate the influence of soil factors on carbon utilization activity of single micro-organisms.
The assay for Rhizobium leguminosarum bv. trifolii in γ-irradiated soil, using the MicroResp(™) system, was optimized for sterility, incubation time, and moisture level. The optimized method was validated with experiments that assessed (i) differences in C utilization of different rhizobia strains and (ii) how this was affected by soil type. Carbon utilization differed among strains of the same species (and symbiovar), but some strains were more responsive to the soil environment than others.
This novel modification of the MicroResp(™) has enabled the scope of carbon-utilization patterns of single strains of bacteria, such as Rh. leguminosarum bv. trifolii, to be studied in soil.
The system is a new tool with applications in microbial ecology adaptable to the study of many culturable bacterial and fungal soil-borne taxa. It will allow measurement of a micro-organism's ability to utilize common C sources released in rhizosphere exudates to be measured in a physical soil background. This knowledge may improve selection efficiency and deployment of commercial microbial inoculants.
本研究旨在开发一种工具，用于探究土壤因素对单一微生物碳利用活性的影响。
使用MicroResp(™)系统对γ射线辐照土壤中的三叶草根瘤菌进行分析，针对无菌性、培养时间和湿度水平进行了优化。通过评估以下实验对优化后的方法进行了验证：(i)不同根瘤菌菌株碳利用的差异，以及(ii)土壤类型对其的影响。同一物种（和共生变种）的不同菌株碳利用情况存在差异，但有些菌株对土壤环境的反应比其他菌株更敏感。
对MicroResp(™)的这种新颖改进能够在土壤中研究单一细菌菌株（如三叶草根瘤菌）的碳利用模式范围。
该系统是一种新工具，可应用于微生物生态学，适用于研究许多可培养的土壤传播细菌和真菌分类群。它将能够在物理土壤背景下测量微生物利用根际分泌物中释放的常见碳源的能力。这些知识可能会提高商业微生物接种剂的选择效率和应用效果。
