Rezapour Saleheh, Bahrami Tayyeb, Hashemzadeh Shahryar, Estiar Mehrdad Asghari, Nemati Masoumeh, Ravanbakhsh Reyhaneh, Feizi Mohammad Ali Hosseinpour, Kafil Hossein Samadi, Pouladi Nasser, Ghojazadeh Morteza, Sakhinia Ebrahim
Clin Lab. 2016;62(3):463-9. doi: 10.7754/clin.lab.2015.150827.
Stanniocalcin-1 (STC1) and nuclear factor (NF)-κB subunit p65 transcription factor are involved in various types of human malignancies. The roles of STC1 and NFκB-p65 in colorectal cancer (CRC) are still not fully understood. We investigated expression levels of NF-κB p65 and STC1 and also correlations between STC1 and NF-κB p65 expression and clinicopathological features in CRC.
Tumor tissue samples were collected from 48 patients with CRC. RT-PCR and Real-time PCR analysis was performed to examine mRNA levels of STC1 and NF-κB p65.
The relative mRNA levels of STC1 and NF-κB p65 were significantly higher in tumor tissues than in adjacent mucosa (p = 0.025 and p = 0.044, respectively). The data also showed that STC1 and NF-κB p65 mRNA levels were not significantly associated with clinicopathological characteristics. In addition, there was no association between expression levels of STC1 and NF-κB p65 in tumor samples.
Our data indicate that STC1 and NF-κBp65 is activated constitutively in colorectal carcinoma tissues, suggesting that activation of these factors might play an important role in colorectal tumorigenesis. Future studies should examine STC1 and NF-κBp65 as a molecular target for the treatment of CRC.
鲟鱼钙蛋白-1（STC1）和核因子（NF）-κB亚基p65转录因子参与多种人类恶性肿瘤。STC1和NFκB-p65在结直肠癌（CRC）中的作用仍未完全明确。我们研究了NF-κB p65和STC1的表达水平，以及STC1与NF-κB p65表达之间的相关性及其与CRC临床病理特征的关系。
收集48例CRC患者的肿瘤组织样本。采用RT-PCR和实时PCR分析检测STC1和NF-κB p65的mRNA水平。
肿瘤组织中STC1和NF-κB p65的相对mRNA水平显著高于相邻黏膜（分别为p = 0.025和p = 0.044）。数据还显示，STC1和NF-κB p65的mRNA水平与临床病理特征无显著相关性。此外，肿瘤样本中STC1和NF-κB p65的表达水平之间无关联。
我们的数据表明，STC1和NF-κBp65在结直肠癌组织中持续激活，提示这些因子的激活可能在结直肠癌发生中起重要作用。未来的研究应将STC1和NF-κBp65作为CRC治疗的分子靶点进行研究。
