Dong Zhen, Zhang Chunming, Zhao Qinli, Huangfu Hui, Xue Xuting, Wen Shuxin, Wu Yongyan, Gao Wei, Wang Binquan
Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
Department of Otolaryngology Head & Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
J Cancer. 2021 May 13;12(13):4049-4063. doi: 10.7150/jca.54221. eCollection 2021.
Bacteria are among the important factors that play a role in the balance of human health, and their relationship with some tumors has been well established. However, the association between bacteria colonizing the vocal cords and glottic laryngeal squamous cell carcinoma (GLSCC) remains unclear. Here, we investigated whether bacterial communities of the vocal cord mucous membrane play a role in the development of GLSCC. We collected tumor tissue and normal adjacent tissue (NAT) samples from 19 GLSCC patients, and the bacterial communities were compared with control samples (control) from 21 vocal cord polyps using 16S rRNA high-throughput pyrosequencing. We detected 41 phyla, 93 classes, 188 orders, 373 families, and 829 genera in the vocal cord mucous membrane. A comparison of the bacterial communities in the NAT samples showed higher α-diversity than in the tumor samples. In the tumor samples, seven groups of bacteria, i.e., the phylum Fusobacteria, the class Fusobacteriia, the order Fusobacteriales, the family Fusobacteriaceae, and the genera , , and , were significantly enriched, as revealed by linear discriminant analysis coupled with effect size measurements (LEfSe). However, bacteria from the phylum Firmicutes were most significantly enriched in the vocal cord polyp tissues. These findings suggest alterations in the bacterial community structure of the vocal cord mucous membrane of GLSCC patients and that seven groups of bacteria are related to GLSCC, indicating that imbalances in bacterial communities increase the risk for the development of GLSCC.
细菌是影响人类健康平衡的重要因素之一，并且它们与某些肿瘤的关系已得到充分证实。然而，定植于声带的细菌与声门型喉鳞状细胞癌（GLSCC）之间的关联仍不明确。在此，我们研究了声带黏膜细菌群落是否在GLSCC的发生发展中起作用。我们收集了19例GLSCC患者的肿瘤组织和正常邻近组织（NAT）样本，并使用16S rRNA高通量焦磷酸测序将细菌群落与21例声带息肉的对照样本（对照组）进行比较。我们在声带黏膜中检测到41个门、93个纲、188个目、373个科和829个属。NAT样本中细菌群落的α多样性高于肿瘤样本。在肿瘤样本中，通过线性判别分析结合效应大小测量（LEfSe）发现，七组细菌，即梭杆菌门、梭杆菌纲、梭杆菌目、梭杆菌科以及属、、和显著富集。然而，厚壁菌门的细菌在声带息肉组织中富集最为显著。这些发现表明GLSCC患者声带黏膜细菌群落结构发生改变，且七组细菌与GLSCC相关，这表明细菌群落失衡增加了GLSCC发生的风险。
