用于检测脂磷壁酸的夹心免疫测定法。

Sandwich immunoassay for the detection of lipoteichoic acid.

作者信息

Tadler M B, Tice G, Wos S M

机构信息

Medical Products Dept., Glasgow Research Lab, E.I. du Pont de Nemours and Co., Wilmington, DE 19898.

出版信息

J Clin Lab Anal. 1989;3(1):21-5. doi: 10.1002/jcla.1860030106.

DOI:10.1002/jcla.1860030106

PMID:2715872

Abstract

A sandwich immunoassay has been developed for the detection of lipoteichoic acid (LTA), a major cell wall constituent of gram-positive bacteria, from whole blood and ISOLATOR supernate. Monoclonal antibodies were produced to purified LTA from Streptococcus mutans, BHT and were further characterized for crossreactivity with gram-positive and negative bacteria and for reactivity to substituted and unsubstituted LTA. Eight monoclonal antibodies were identified that reacted exclusively with gram-positive bacteria. Those antibodies able to capture 3H-LTA were chosen to develop a sandwich immunoassay. The assay has a sensitivity of 0.2 ng LTA/mL in PBS, 0.5 ng/mL in whole blood and 2.0 ng/mL in whole blood that has been processed through the ISOLATOR. Further development of this assay may lead to the rapid detection of LTA from body fluids.

摘要

已开发出一种夹心免疫测定法，用于从全血和ISOLATOR上清液中检测脂磷壁酸（LTA），它是革兰氏阳性菌细胞壁的主要成分。针对变形链球菌、BHT的纯化LTA制备了单克隆抗体，并进一步对其与革兰氏阳性菌和阴性菌的交叉反应性以及对取代和未取代LTA的反应性进行了表征。鉴定出了8种仅与革兰氏阳性菌反应的单克隆抗体。选择那些能够捕获3H-LTA的抗体来开发夹心免疫测定法。该测定法在PBS中的灵敏度为0.2 ng LTA/mL，在全血中为0.5 ng/mL，在经ISOLATOR处理的全血中为2.0 ng/mL。该测定法的进一步开发可能会实现从体液中快速检测LTA。