Hamada S, Furuta T, Okahashi N, Nisizawa T, Yamamoto T, Chiba J
Microbiol Immunol. 1984;28(9):1009-21. doi: 10.1111/j.1348-0421.1984.tb00757.x.
A hybrid cell line, 3G6, producing monoclonal antibody (mAb) against the polyglycerophosphate (PGP) backbone of lipoteichoic acids has been derived by the polyethylene glycol-induced fusion of mouse myeloma cells and spleen cells from mice immunized with partially purified glucosyltransferase from culture supernatant of Streptococcus mutans strain 6715. Immunodiffusion tests and ELISA revealed that the antibody reacted with purified PGP from group A Streptococcus pyogenes strain Sv as well as crude phenol-water and saline extracts of various gram-positive bacteria except for a few species such as biotype B S. sanguis, Micrococcus sp., and Actinomyces viscosus. Whole cells of serotype b S. mutans and Staphylococcus epidermidis were agglutinated upon addition of 3G6 mAb, while those of most other species were not significantly affected by this procedure. A hapten inhibition study showed that glycerophosphate was only a potent inhibitor of passive hemagglutination reactions between LTA coated sheep erythrocytes and 3G6 mAb.
一种杂交细胞系3G6已通过聚乙二醇诱导的小鼠骨髓瘤细胞与来自用变形链球菌6715株培养上清液中部分纯化的葡糖基转移酶免疫的小鼠脾细胞融合而获得，该细胞系产生针对脂磷壁酸多聚甘油磷酸（PGP）主链的单克隆抗体（mAb）。免疫扩散试验和酶联免疫吸附测定表明，该抗体与化脓性链球菌A组菌株Sv的纯化PGP以及除少数菌种（如血链球菌生物型B、微球菌属和粘性放线菌）外的各种革兰氏阳性菌的粗酚水提取物和盐水提取物发生反应。加入3G6 mAb后，变形链球菌b血清型和表皮葡萄球菌的全细胞发生凝集，而大多数其他菌种的全细胞在此过程中未受到明显影响。半抗原抑制研究表明，甘油磷酸只是脂磷壁酸包被的绵羊红细胞与3G6 mAb之间被动血凝反应的有效抑制剂。
