Kumar Raj, Gopalakrishnan A, Divya P R, Basheer V S, Singh Rajeev K, Mohindra Vindya, Lal Kuldeep K, Jena J K
a Peninsular and Marine Fish Genetic Resources Centre (PMFGR), National Bureau of Fish Genetic Resources , CMFRI Campus , Kochi , Kerala , India.
b Central Marine Fisheries Research Institute , Kochi , Kerala , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jul;28(4):602-605. doi: 10.3109/24701394.2016.1149829. Epub 2016 Mar 8.
Macrobrachium rosenbergii, giant freshwater prawn, is one of the most commercially important crustaceans. In the present study, primers for ATPase 6/8 region of mt-DNA were designed and successfully amplified (827 bp) in the species. The nucleotide variation in ATPase 6/8 gene revealed the population structuring in natural populations of M. rosenbergii in Indian waters. A total of 35 haplotypes were observed in 93 individuals collected from different locations. Low nucleotide diversity and high haplotype diversity were noticed for the ATPase 6/8 gene. Significant pairwise F and, haplotype network indicated occurrence of distinct populations. Observed mismatch distribution and Tajima's D test suggested demographical stability of giant freshwater prawn. The genetic stock structure revealed in this study will be helpful for conservation and management of stocks of M. rosenbergii in Indian waters.
罗氏沼虾，即淡水大对虾，是最重要的商业养殖甲壳类动物之一。在本研究中，设计了线粒体DNA的ATPase 6/8区域引物，并在该物种中成功扩增出（827 bp）片段。ATPase 6/8基因的核苷酸变异揭示了印度水域罗氏沼虾自然种群的群体结构。从不同地点采集的93个个体中共观察到35种单倍型。ATPase 6/8基因呈现出低核苷酸多样性和高单倍型多样性。显著的成对F值和单倍型网络表明存在不同的种群。观察到的错配分布和Tajima's D检验表明淡水大对虾的种群结构稳定。本研究揭示的遗传种群结构将有助于印度水域罗氏沼虾种群的保护和管理。
