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大豆茎潜蝇(双翅目:潜蝇科)的完整线粒体基因组

Complete mitochondrial genome of the soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae).

作者信息

Arnemann Jonas André, Walsh Tom K, Gordon Karl H J, Brier Hugh, Guedes Jerson Vanderlei Carús, Tay Wee Tek

机构信息

a Black Mountain Laboratories, CSIRO , Canberra , ACT , Australia.

b Department of Crop Protection , Federal University of Santa Maria (Universidade Federal de Santa Maria) , Santa Maria, Rio Grande do Sul State , Brazil , and.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4534-4535. doi: 10.3109/19401736.2015.1101550. Epub 2016 Mar 22.

Abstract

We report the complete mitochondrial DNA genome of the soybean stem fly (SSF) Melanagromyza sojae from Brazil Santa Catarina state based on Illumina MiSeq sequence data. The estimated mitogenome is 15 475 base pairs (bp) (KT597923), with 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, and 22 tRNAs, and an estimated 579 bp AT-rich control region. Similar to other insects, the SSF mitogenome is A-T bias with 40.9% A, 36.7% T, 13.6% C, and 8.8% G. Molecular characterization of SSF mitogenome will facilitate the development of effective molecular markers, and robust and rapid identification of suspected biosecurity incursions and field infestations of this insect pest.

摘要

我们基于Illumina MiSeq序列数据,报道了来自巴西圣卡塔琳娜州的大豆茎蝇(SSF)大豆黑潜蝇的完整线粒体DNA基因组。估计的线粒体基因组为15475个碱基对(bp)(KT597923),包含13个蛋白质编码基因(PCG)、两个核糖体RNA(rRNA)基因、22个转运RNA(tRNA),以及一个估计为579bp的富含AT的控制区。与其他昆虫相似,大豆茎蝇线粒体基因组具有A-T偏向性,其中A占40.9%,T占36.7%,C占13.6%,G占8.8%。大豆茎蝇线粒体基因组的分子特征将有助于开发有效的分子标记,以及对这种害虫的疑似生物安全入侵和田间侵染进行可靠且快速的鉴定。

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