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烟草天蛾(鳞翅目:夜蛾科)一个形态种的完整线粒体DNA基因组

The complete mitochondrial DNA genome of a Chloridea (Heliothis) subflexa (Lepidoptera: Noctuidae) morpho-species.

作者信息

de Souza Bruna Raquel, Tay Wee Tek, Czepak Cecilia, Elfekih Samia, Walsh Thomas Kieran

机构信息

a CSIRO, Black Mountain Laboratories , Canberra , ACT , Australia.

b Centro De Ciências Biológicas, Universidade Federal De Santa Catarina (UFSC) , Florianópolis, SC , Brazil , and.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4532-4533. doi: 10.3109/19401736.2015.1101549. Epub 2015 Nov 5.

Abstract

We investigate the complete mitogenome of a pheromone-trapped morpho-species of Chloridea subflexa from Brazil (initially identified by the Sanger sequencing of partial mtCOI gene) as 15 323 bp (KT598688) via next generation sequencing platform. The mitogenome has an A/T rich base composition (A: 40.4%; T: 40.3%; C: 11.5%; G: 7.8%), and included 13 protein-coding genes (PCGs), 22 tRNAs, 2 ribosomal RNAs and a putative replication region (ca. 323 bp). All PCGs start with a methionine (M) amino acid except the COI gene which has an arginine (R). The trnL2 and trn-Lys genes were partially embedded within the COII gene, while the trn-His gene was completely embedded within the ND4 gene. All PCGs ends with the "TAA" stop codon except ND3 which has a "TAG" stop codon.

摘要

我们通过下一代测序平台研究了来自巴西的一种经性信息素诱捕的亚曲绿夜蛾形态种的完整线粒体基因组(最初通过部分线粒体细胞色素氧化酶亚基I基因的桑格测序鉴定),其长度为15323 bp(登录号KT598688)。该线粒体基因组具有富含A/T的碱基组成(A:40.4%;T:40.3%;C:11.5%;G:7.8%),包含13个蛋白质编码基因(PCGs)、22个tRNA、2个核糖体RNA和一个假定的复制区域(约323 bp)。除细胞色素氧化酶亚基I(COI)基因起始密码子为精氨酸(R)外,所有PCGs均以甲硫氨酸(M)起始。trnL2和trn-Lys基因部分嵌入细胞色素氧化酶亚基II(COII)基因内,而trn-His基因完全嵌入烟酰胺腺嘌呤二核苷酸脱氢酶亚基4(ND4)基因内。除ND3基因的终止密码子为“TAG”外,所有PCGs均以“TAA”终止密码子结束。

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