Gaikwad Swapnil, Warudkar Ashwin, Shouche Yogesh
a Microbial Culture Collection (MCC) , Pune , Maharashtra , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Sep;28(5):638-644. doi: 10.3109/24701394.2016.1166219. Epub 2016 Apr 26.
DNA barcoding has emerged as an additional tool for taxonomy and as an aid to taxonomic impediments. Due to their extensive morphological variation, spiders are taxonomically challenging. Therefore, all over the world, attempts are being made to DNA barcode species of spiders. Till now no attempts were made to DNA barcode Indian spiders despite their rich diversity. We have generated DNA barcodes for 60 species (n = 112) of spiders for the first time from India. Although only 17 species were correctly identified at the species level, DNA barcoding correctly discriminated 99% of the species studied here. We have also found high intraspecies nucleotide divergence in Plexippus paykulli suggesting cryptic diversity that needs to be studied in detail. Our study also showed non-specific amplification of the Cytochrome Oxidase I (COI) gene of endosymbiont bacteria Wolbachia. However, these cases are very rare and could be resolved by the use of modified or group specific primers.
DNA条形码已成为分类学的一种辅助工具，并有助于克服分类学障碍。蜘蛛由于其广泛的形态变异，在分类学上具有挑战性。因此，世界各地都在尝试对蜘蛛物种进行DNA条形码分析。尽管印度蜘蛛种类丰富，但此前从未有人尝试对其进行DNA条形码分析。我们首次从印度为60种（n = 112）蜘蛛生成了DNA条形码。虽然在物种水平上仅正确鉴定出17种，但DNA条形码正确区分了此处研究的99%的物种。我们还发现巴氏蝇虎（Plexippus paykulli）存在较高的种内核苷酸差异，这表明存在需要详细研究的隐性多样性。我们的研究还显示内共生细菌沃尔巴克氏体（Wolbachia）的细胞色素氧化酶I（COI）基因存在非特异性扩增。然而，这些情况非常罕见，可以通过使用改良引物或组特异性引物来解决。
