Department of Zoology, Modern College, Shivajinagar, Pune, 411005 Maharashtra, India.
Mol Biol Rep. 2012 Mar;39(3):2375-83. doi: 10.1007/s11033-011-0988-7. Epub 2011 Jun 14.
We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.
我们已经通过使用线粒体基因细胞色素 c 氧化酶亚基 I 的 650bp 序列检查了 DNA 条形码在鉴定来自印度西高止山脉的蛱蝶物种方面的实用性。在这里研究的物种之间存在明显的 DNA 条形码间隙（即种内和种间核苷酸差异）。当我们的序列与来自不同地理区域的同种提交的序列进行比较时，观察到了 9 个种内核苷酸深度分歧的情况。尽管如此，NJ（邻接法）聚类分析成功地区分了所有物种。观察到的种内核苷酸深度分歧的情况肯定需要进一步研究。
