Ahmed Hanaa H, Toson Elshahat A, El-Mezayen Hatem A, Rashed Laila A, Elsherbiny Eslam S
Hormones Department, National Research Centre, Dokki, Giza, Egypt.
Chemistry Department, Faculty of Science, Damietta University, Damietta, Egypt.
Nephrology (Carlton). 2017 Jul;22(7):531-540. doi: 10.1111/nep.12812.
The current study sought to clarify the role of bone marrow derived mesenchymal stem cells (BM-MSCs) and adipose tissue derived mesenchymal stem cells (AD-MSCs) in repressing nephropathy in the experimental model. Moreover, the aim of this work was extended to compare between stem cells role and angiotensin converting enzyme inhibitor in kidney repair.
Isolation and preparation of MSCs culture, flow cytometry using CD34, CD44 and CD105 cell surface markers, biochemical analyses for determination of serum creatinine, urea, transforming growth factor β (TGF-β), cystatin C (CYS-C) and urinary N-Acetyl-ß-D-Glucosaminidase (UNAG), and histopathological investigation of kidney tissue sections were performed.
The results of the present study revealed that single intravenous infusion of MSCs either derived from bone marrow or adipose tissue was able to enhance renal reparative processes through significantly decreased serum creatinine, urea, TGF-β and CYS-C levels as well as UNAG level and significantly increase glomerular filtration rate. Additionally, the histopathological investigations of kidney tissues showed that MSCs have significant regenerative effects as evidenced by the decrease in focal inflammatory cells infiltration, focal interstitial nephritis and congested glomeruli as well as degenerated tubules.
The current data provided distinct evidence about the favourable impact of AD-MSCs and BM-MSCs in attenuation of cyclosporine-induced nephropathy in rats through their ability to promote functional and structural kidney repair via transdifferentiation.
本研究旨在阐明骨髓间充质干细胞（BM-MSCs）和脂肪组织来源的间充质干细胞（AD-MSCs）在实验模型中抑制肾病的作用。此外，本研究的目的还扩展到比较干细胞与血管紧张素转换酶抑制剂在肾脏修复中的作用。
进行间充质干细胞培养的分离与制备、使用CD34、CD44和CD105细胞表面标志物的流式细胞术、测定血清肌酐、尿素、转化生长因子β（TGF-β）、胱抑素C（CYS-C）和尿N-乙酰-β-D-氨基葡萄糖苷酶（UNAG）的生化分析，以及对肾组织切片进行组织病理学研究。
本研究结果显示，单次静脉输注来源于骨髓或脂肪组织的间充质干细胞能够通过显著降低血清肌酐、尿素、TGF-β和CYS-C水平以及UNAG水平，并显著提高肾小球滤过率来增强肾脏修复过程。此外，肾组织的组织病理学研究表明，间充质干细胞具有显著的再生作用，表现为局灶性炎性细胞浸润、局灶性间质性肾炎、肾小球充血以及肾小管变性减少。
目前的数据提供了明确的证据，表明AD-MSCs和BM-MSCs通过其通过转分化促进肾脏功能和结构修复的能力，对环孢素诱导的大鼠肾病具有减轻作用。
