基于免疫聚合酶链反应检测分枝杆菌抗原85B、早期分泌性抗原靶6和索状因子混合物对结核病进行诊断。
Diagnosis of tuberculosis based on the detection of a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor by immuno-PCR.
作者信息
Mehta Promod K, Singh Netrapal, Dharra Renu, Dahiya Bhawna, Sharma Suman, Sheoran Abhishek, Gupta Krishna B, Chaudhary Dhruva, Mehta Neeru, Varma-Basil Mandira
机构信息
Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, 124001 Haryana, India.
出版信息
J Microbiol Methods. 2016 Aug;127:24-27. doi: 10.1016/j.mimet.2016.05.003. Epub 2016 May 7.
Attempts were made to enhance the sensitivity of immuno-PCR assay based on the detection of cocktail of mycobacterial antigen 85B (Rv1886c), ESAT-6 (Rv3875) and cord factor (trehalose 6,6'-dimycolate) in pulmonary and extrapulmonary TB patients. Detection of Ag85B was found to be superior to the detection of cocktail in TB patients.
研究人员尝试通过检测肺结核和肺外结核患者体内的结核分枝杆菌抗原85B（Rv1886c）、早期分泌性抗原靶6（ESAT-6，Rv3875）和索状因子（海藻糖6,6'-二分枝菌酸）混合物来提高免疫PCR检测法的灵敏度。结果发现，在结核病患者中，检测抗原85B的效果优于检测该混合物。
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