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动物结核病的诊断及非特异性结核菌素反应的鉴别

Diagnostics of tuberculosis and differentiation of nonspecific tuberculin reactions in animals.

作者信息

Basybekov Sovetzhan Z, Bazarbayev Marat B, Yespembetov Bolat A, Mussaeva Assiya, Kanatbayev Serik G, Romashev Kanapya M, Dossanova Aigul K, Yelekeyev Tokseiit A, Akmatova Elmira K, Syrym Nazym S

机构信息

Kazakh Research Veterinary Institute, Gvardeiskiy, Kazakhstan.

Research Institute of Biological Safety Problems, Gvardeiskiy, Kazakhstan.

出版信息

Braz J Microbiol. 2018 Apr-Jun;49(2):329-335. doi: 10.1016/j.bjm.2017.07.004. Epub 2017 Oct 25.

DOI:10.1016/j.bjm.2017.07.004
PMID:29153452
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5913827/
Abstract

Tuberculosis is a serious disease of humans and animals, caused by bacteria of the Mycobacterium genus. This leads to complications in the life of the sick person, and subsequently to death. The cattle, who have been diagnosed with this bacterium, are usually sent to the slaughter, with the result that their livestock is reduced. Mycobacteriosis is also a disease, after determining which cattle are most often sent to slaughter. Such a reduction in livestock numbers has a negative effect on the economy. Of the 300 samples from the animals, 25 cultures of atypical bacteria responding to tuberculin were isolated. A series of tests - intravenous tuberculin test, ophthalmic test, palpebral test, "ZhAT" test, showed that most of the tuberculosis changes in cattle were found in regional lymph nodes more often than in internal organs. In healthy for tuberculosis cows, at the age of 4-9 years, seasonal nonspecific sensitivity to tuberculin is observed. Implementation of the developed express method of glutaraldehyde test on farms in healthy tuberculosis will speed up the diagnosis of tuberculosis and mycobacteriosis in animals that reacted to tuberculin and will exclude short-term nonspecific sensitization of their organism to tuberculin. The introduction of this methodology can be used to diagnose and clearly differentiate the diagnoses of "tuberculosis" and "mycobacteriosis" in cattle. This will cure part of the livestock and reduce the amount of slaughter.

摘要

结核病是一种严重的人畜共患疾病,由分枝杆菌属细菌引起。这会导致患者生活出现并发症,进而导致死亡。被诊断出感染这种细菌的牛通常会被送去屠宰,结果其牲畜数量减少。分枝杆菌病也是一种疾病,确定感染后牛最常被送去屠宰。牲畜数量的这种减少对经济有负面影响。从动物身上采集的300份样本中,分离出了25株对结核菌素呈反应的非典型细菌培养物。一系列检测——静脉注射结核菌素试验、眼试验、睑试验、“ZhAT”试验表明,牛的结核病病变大多更常出现在局部淋巴结而非内脏器官。在4至9岁的健康无结核病奶牛中,观察到对结核菌素的季节性非特异性敏感性。在健康无结核病的农场实施已开发的戊二醛快速检测方法,将加快对结核菌素呈反应的动物结核病和分枝杆菌病的诊断,并排除其机体对结核菌素的短期非特异性致敏。引入这种方法可用于诊断和明确区分牛的“结核病”和“分枝杆菌病”诊断。这将治愈部分牲畜并减少屠宰量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/e2fa8c59bb4c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/151da057ac8f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/b1c9eaa2d711/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/e2fa8c59bb4c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/151da057ac8f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/b1c9eaa2d711/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca15/5913827/e2fa8c59bb4c/gr3.jpg

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本文引用的文献

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Iran J Med Sci. 2016 Jul;41(4):296-304.
2
Diagnosis of tuberculosis based on the detection of a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor by immuno-PCR.基于免疫聚合酶链反应检测分枝杆菌抗原85B、早期分泌性抗原靶6和索状因子混合物对结核病进行诊断。
J Microbiol Methods. 2016 Aug;127:24-27. doi: 10.1016/j.mimet.2016.05.003. Epub 2016 May 7.
3
Modelling the role of multi-transmission routes in the epidemiology of bovine tuberculosis in cattle and buffalo populations.
模拟多传播途径在牛和水牛群体中牛结核病流行病学中的作用。
Math Biosci. 2016 Jul;277:47-58. doi: 10.1016/j.mbs.2016.04.003. Epub 2016 Apr 20.
4
Attitudes about tuberculosis prevention in the elimination phase: a survey among physicians in Germany.消除阶段对结核病预防的态度:德国医生的一项调查
PLoS One. 2014 Nov 13;9(11):e112681. doi: 10.1371/journal.pone.0112681. eCollection 2014.
5
Modeling of novel diagnostic strategies for active tuberculosis - a systematic review: current practices and recommendations.活动性肺结核新型诊断策略建模——系统评价:当前实践与建议
PLoS One. 2014 Oct 23;9(10):e110558. doi: 10.1371/journal.pone.0110558. eCollection 2014.
6
A multi-site evaluation of innovative approaches to increase tuberculosis case notification: summary results.增加结核病病例通报创新方法的多地点评估:总结结果
PLoS One. 2014 Apr 10;9(4):e94465. doi: 10.1371/journal.pone.0094465. eCollection 2014.
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Overview and phylogeny of Mycobacterium tuberculosis complex organisms: implications for diagnostics and legislation of bovine tuberculosis.结核分枝杆菌复合群生物的概述与系统发育:对牛结核病诊断和立法的影响
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Amplified Mycobacterium tuberculosis direct test for diagnosing tuberculous pleurisy--a diagnostic accuracy study.扩增分枝杆菌结核直接检测诊断结核性胸膜炎的诊断准确性研究。
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