Maekura R, Nakagawa M, Nakamura Y, Hiraga T, Yamamura Y, Ito M, Ueda E, Yano S, He H, Oka S
Toneyama National Hospital, Osaka, Japan.
Am Rev Respir Dis. 1993 Oct;148(4 Pt 1):997-1001. doi: 10.1164/ajrccm/148.4_Pt_1.997.
Immunoglobulin G (IgG) antibodies against purified cord factor (trehalose-6,6'-dimycolate) prepared from Mycobacterium tuberculosis H37Rv were determined by enzyme-linked immunosorbent assay (ELISA), and its diagnostic usefulness was evaluated. Serum specimens from 65 patients with active pulmonary tuberculosis, 58 patients with inactive pulmonary tuberculosis, 36 patients with diseases other than tuberculosis, and 66 healthy adults were examined. Patients with active pulmonary tuberculosis showed significantly higher titers of IgG antibodies against cord factor than did other groups (p < 0.001). The antibody titer greater than 0.29 in absorption difference (492 to 630 nm) of 160-times diluted serum was set as positive in ELISA. For patients with active and untreated pulmonary tuberculosis, the ELISA had a sensitivity of 81% and a specificity of 96%. From these results, it was concluded that the detection of IgG antibodies against cord factor is useful for serodiagnosis of active pulmonary tuberculosis. It was also indicated that the anticord factor antibody titers decline to the normal level as a result of antituberculosis chemotherapy.
采用酶联免疫吸附测定法（ELISA）测定了针对从结核分枝杆菌H37Rv制备的纯化索状因子（海藻糖 - 6,6'-二霉菌酸酯）的免疫球蛋白G（IgG）抗体，并评估了其诊断价值。检测了65例活动性肺结核患者、58例非活动性肺结核患者、36例非结核疾病患者和66例健康成年人的血清标本。活动性肺结核患者针对索状因子的IgG抗体滴度显著高于其他组（p < 0.001）。在ELISA中，将160倍稀释血清在492至630 nm吸光度差值大于0.29的抗体滴度设定为阳性。对于未经治疗的活动性肺结核患者，ELISA的敏感性为81%，特异性为96%。从这些结果得出结论，检测针对索状因子的IgG抗体对活动性肺结核的血清学诊断有用。还表明，抗索状因子抗体滴度因抗结核化疗而降至正常水平。
