Ferrer Albert, Arró Monserrat, Manzano David, Altabella Teresa
Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) (CSIC-IRTA-UAB-UB), Campus UAB, Bellaterra-Cerdanyola del Vallès, 08193, Barcelona, Spain.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Barcelona, 08028, Barcelona, Spain.
Adv Exp Med Biol. 2016;896:263-85. doi: 10.1007/978-3-319-27216-0_17.
The first transgenes were introduced in a plant genome more than 30 years ago. Since then, the capabilities of the plant scientific community to engineer the genome of plants have progressed at an unparalleled speed. Plant genetic engineering has become a central technology that has dramatically incremented our basic knowledge of plant biology and has enabled the translation of this knowledge into a number of increasingly complex and sophisticated biotechnological applications, which in most cases rely on the simultaneous co-expression of multiple recombinant proteins from different origins. To meet the new challenges of modern plant biotechnology, the plant scientific community has developed a vast arsenal of innovative molecular tools and genome engineering strategies. In this chapter we review a variety of tools, technologies, and strategies developed to transfer and simultaneously co-express multiple transgenes and proteins in a plant host. Their potential advantages, disadvantages, and future prospects are also discussed.
30多年前，首批转基因被导入植物基因组。从那时起，植物科学界对植物基因组进行工程改造的能力以空前的速度发展。植物基因工程已成为一项核心技术，极大地增加了我们对植物生物学的基础知识，并使这些知识转化为许多日益复杂和精密的生物技术应用，在大多数情况下，这些应用依赖于同时共表达来自不同来源的多种重组蛋白。为应对现代植物生物技术的新挑战，植物科学界开发了大量创新的分子工具和基因组工程策略。在本章中，我们将综述为在植物宿主中转移并同时共表达多个转基因和蛋白质而开发的各种工具、技术和策略。还将讨论它们的潜在优势、劣势和未来前景。
