White Richard M
Memorial Sloan Kettering Cancer Center, 415 East 68th Street, New York, NY, 10065, USA.
Adv Exp Med Biol. 2016;916:125-45. doi: 10.1007/978-3-319-30654-4_6.
The zebrafish has emerged as an important model for studying cancer biology. Identification of DNA, RNA and chromatin abnormalities can give profound insight into the mechanisms of tumorigenesis and the there are many techniques for analyzing the genomes of these tumors. Here, I present an overview of the available technologies for analyzing tumor genomes in the zebrafish, including array based methods as well as next-generation sequencing technologies. I also discuss the ways in which zebrafish tumor genomes can be compared to human genomes using cross-species oncogenomics, which act to filter genomic noise and ultimately uncover central drivers of malignancy. Finally, I discuss downstream analytic tools, including network analysis, that can help to organize the alterations into coherent biological frameworks that can then be investigated further.
斑马鱼已成为研究癌症生物学的重要模型。DNA、RNA和染色质异常的鉴定能够深入洞察肿瘤发生的机制，并且有许多技术可用于分析这些肿瘤的基因组。在此，我将概述用于分析斑马鱼肿瘤基因组的现有技术，包括基于阵列的方法以及新一代测序技术。我还将讨论如何利用跨物种肿瘤基因组学将斑马鱼肿瘤基因组与人类基因组进行比较，这种方法有助于过滤基因组噪音并最终揭示恶性肿瘤的核心驱动因素。最后，我将讨论下游分析工具，包括网络分析，这些工具有助于将改变整合到连贯的生物学框架中，以便进一步研究。
