Monzani Paulo S, Adona Paulo R, Ohashi Otávio M, Meirelles Flávio V, Wheeler Matthew B
a Centro de Ciências Biológicas e da Saúde , Universidade Norte do Paraná , Londrina , Paraná , Brazil.
b Departamento de Ciências Básicas , Universidade de São Paulo , Pirassununga , São Paulo , Brazil.
Bioengineered. 2016 Apr;7(3):123-31. doi: 10.1080/21655979.2016.1171429. Epub 2016 May 11.
The use of recombinant proteins has increased in diverse commercial sectors. Various systems for protein production have been used for the optimization of production and functional protein expression. The mammary gland is considered to be a very interesting system for the production of recombinant proteins due to its high level of expression and its ability to perform post-translational modifications. Cows produce large quantities of milk over a long period of lactation, and therefore this species is an important candidate for recombinant protein expression in milk. However, transgenic cows are more difficult to generate due to the inefficiency of transgenic methodologies, the long periods for transgene detection, recombinant protein expression and the fact that only a single calf is obtained at the end of each pregnancy. An increase in efficiency for transgenic methodologies for cattle is a big challenge to overcome. Promising methodologies have been proposed that can help to overcome this obstacle, enabling the use of transgenic cattle as bioreactors for protein production in milk for industry.
重组蛋白在不同商业领域的应用日益增加。各种蛋白质生产系统已被用于优化生产和功能性蛋白表达。乳腺被认为是生产重组蛋白的一个非常有吸引力的系统，因为它具有高水平的表达能力以及进行翻译后修饰的能力。奶牛在长时间的泌乳期内会产出大量牛奶，因此该物种是在牛奶中表达重组蛋白的重要候选对象。然而，由于转基因方法效率低下、转基因检测、重组蛋白表达所需时间长，且每次怀孕最终只能产下一头小牛，所以培育转基因奶牛更加困难。提高牛转基因方法的效率是一个亟待克服的巨大挑战。目前已经提出了一些有前景的方法，这些方法有助于克服这一障碍，使转基因奶牛能够作为生物反应器用于工业生产牛奶中的蛋白。
