Karsdon J, van Rijn J, Berger H, Natarajan A T
Department of Pediatrics, University Hospital of Leiden, The Netherlands.
Mutat Res. 1989 May;226(1):13-9. doi: 10.1016/0165-7992(89)90087-0.
We have examined lymphocytes from human preterm (PT) and fullterm (FT) babies for an effect of gestational age (GA) on chromosomal aberrations either occurring spontaneously or induced by treatment with X-rays (1 Gy) alone; or with caffeine (1,3,7-trimethylxanthine) supplementation (5 X 10(-4) M), in comparison to the lymphocytes of healthy adults (AD). Percent of abnormal cells (%Abn) was used as an indicator of chromosome sensitivity to the different treatments. PT babies had significantly higher spontaneous and X-ray-induced %Abn values than AD, but were comparable to FT. After X-irradiation + caffeine the yield of aberrations in any 2 groups was not significantly different. Chromosomal sensitivity may result from factors other than GA. This in vitro model may permit study of the mechanisms of chromosomal damage repair and prevention of free radical damage of DNA during the perinatal period.
我们检测了人类早产（PT）和足月（FT）婴儿的淋巴细胞，以研究胎龄（GA）对自发出现或仅经X射线（1 Gy）处理；或与补充咖啡因（1,3,7-三甲基黄嘌呤，5×10⁻⁴ M）相比，健康成年人（AD）淋巴细胞所诱导的染色体畸变的影响。异常细胞百分比（%Abn）用作染色体对不同处理敏感性的指标。早产婴儿的自发和X射线诱导的%Abn值显著高于成年人，但与足月婴儿相当。X射线照射 + 咖啡因处理后，任何两组的畸变率均无显著差异。染色体敏感性可能由胎龄以外的因素导致。这种体外模型可能有助于研究围产期DNA自由基损伤的染色体损伤修复和预防机制。
