Wang Ningli, Wei Jianteng, Liu Yewei, Pei Dong, Hu Qingping, Wang Yu, Di Duolong
a Institute of Nutrition and Food Hygiene, School of Public Health, Lanzhou University , Lanzhou , China ;
b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province , Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou , China ;
Biomarkers. 2016 Jul;21(5):449-57. doi: 10.3109/1354750X.2016.1153720. Epub 2016 May 10.
Oxidative stress has a close relationship with various pathologic physiology phenomena and the potential biomarkers of oxidative stress may provide evidence for clinical diagnosis or disease prevention. Metabolomics was employed to identify the potential biomarkers of oxidative stress. High-performance liquid chromatography-diode array detector, mass spectrometry and partial least squares discriminate analysis were used in this study. The 10, 15 and 13 metabolites were considered to discriminate the model group, vitamin E-treated group and l-glutathione-treated group, respectively. Some of them have been identified, namely, malic acid, vitamin C, reduced glutathione and tryptophan. Identification of other potential biomarkers should be conducted and their physiological significance also needs to be elaborated.
氧化应激与多种病理生理现象密切相关，氧化应激的潜在生物标志物可为临床诊断或疾病预防提供依据。采用代谢组学方法来识别氧化应激的潜在生物标志物。本研究使用了高效液相色谱 - 二极管阵列检测器、质谱和偏最小二乘判别分析。分别有10种、15种和13种代谢物被认为可区分模型组、维生素E治疗组和L - 谷胱甘肽治疗组。其中一些已被鉴定出来，即苹果酸、维生素C、还原型谷胱甘肽和色氨酸。还应进行其他潜在生物标志物的鉴定，其生理意义也有待阐述。
