Salahshoor Mohammad Reza, Khashiadeh Mojtaba, Roshankhah Shiva, Kakabaraei Seyran, Jalili Cyrus
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
Students research committee, Kermanshah university of Medical Sciences. Daneshgah Ave, Taghbostan, Kermanshah, I.R. Iran.
Res Pharm Sci. 2016 Mar-Apr;11(2):120-9.
Crocin, a bioactive molecule of saffron can be purely isolated from the saffron extract. It has different pharmacological effects such as antioxidant and anticancer activities. Morphine is an opioid analgesic drug. It is mainly metabolized in liver and causes devastating effects. It can increase the generation of free radicals. This study was designed to evaluate the protective role of crocin against morphine-induced toxicity in the mouse liver. In this study, various doses of crocin (12.5, 25 and 50 mg/kg) and crocin plus morphine were administered interaperitoneally once daily to 48 male mice for 20 consecutive days. These mice were randomly assigned to 8 groups of 6 each. The liver weight and histology, aspartate amino transferase, alanine aminotransferase, alkaline phosphatase (ALP) and serum nitric oxide levels were studied. The results indicated that morphine administration significantly decreased liver weight and increased the mean diameter of hepatocyte, central hepatic vein diameters, liver enzyme levels, and blood serum nitric oxide level compared to saline group (P<0.05). However, crocin administration significantly boosted liver weight and decreased the mean diameter of hepatocyte, central hepatic vein, liver enzymes and nitric oxide levels in all groups compared to the group received morphine alone (P<0.05). It seems that crocin administration could protect the liver damage induced by morphine. The antioxidant effect of crocin may be a major reason for its positive impact on liver parameters.
藏红花素是藏红花的一种生物活性分子，可从藏红花提取物中纯分离得到。它具有不同的药理作用，如抗氧化和抗癌活性。吗啡是一种阿片类镇痛药。它主要在肝脏中代谢，并产生破坏性影响。它会增加自由基的产生。本研究旨在评估藏红花素对吗啡诱导的小鼠肝脏毒性的保护作用。在本研究中，将不同剂量的藏红花素（12.5、25和50毫克/千克）以及藏红花素加吗啡每天腹腔注射一次，连续20天给予48只雄性小鼠。这些小鼠被随机分为8组，每组6只。研究了肝脏重量和组织学、天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶（ALP）和血清一氧化氮水平。结果表明，与生理盐水组相比，给予吗啡显著降低了肝脏重量，增加了肝细胞平均直径、肝中央静脉直径、肝酶水平和血清一氧化氮水平（P<0.05）。然而，与单独接受吗啡的组相比，给予藏红花素显著提高了所有组的肝脏重量，并降低了肝细胞平均直径、肝中央静脉、肝酶和一氧化氮水平（P<0.05）。似乎给予藏红花素可以保护吗啡诱导的肝损伤。藏红花素的抗氧化作用可能是其对肝脏参数产生积极影响的主要原因。
