Abozaid Khaled G A, Aly Mona M, Abdel-Moneim Ahmed S, El-Kady Magdy F
Institute of Animal Health, Fayoum, 63511, Egypt.
Institute of Animal Health, Dokki, Giza, 12618, Egypt.
Trop Anim Health Prod. 2016 Aug;48(6):1221-6. doi: 10.1007/s11250-016-1079-5. Epub 2016 May 12.
Highly pathogenic avian influenza subtype H5N1 represents a threat to the poultry industry and human health worldwide. Inapparently infected birds are suspected to play an essential role in the spread of avian influenza virus. In the current study, a total of 25,646 samples (16,185 chicken, 4696 ducks, 1633 geese and 3132 turkeys) from apparently healthy birds were screened for the presence of positive samples for H5N1 during 2009-2014. The samples were examined by reverse transcriptase real-time polymerase chain reaction (rRT-PCR) for M, H5 and N1 genes of avian influenza viruses. The results revealed that the HPAI H5N1 existed in an inapparent manner in ducks (4.68 %), geese (4.10 %), chickens (2.48 %) and turkeys (2.29 %). The current finding highlights the serious impact of such type on birds in the epidemiology of H5N1 in birds, animals and humans. It also highlights the existence of another reason other than vaccination that contributes to the widespread of inapparent infection of H5N1 in Egypt.
高致病性禽流感H5N1亚型对全球家禽业和人类健康构成威胁。疑似隐性感染的禽类在禽流感病毒传播中起着重要作用。在本研究中，2009年至2014年期间，对来自外观健康禽类的总共25646份样本（16185份鸡样本、4696份鸭样本、1633份鹅样本和3132份火鸡样本）进行了H5N1阳性样本筛查。通过逆转录实时聚合酶链反应（rRT-PCR）检测样本中禽流感病毒的M、H5和N1基因。结果显示，H5N1高致病性禽流感在鸭（4.68%）、鹅（4.10%）、鸡（2.48%）和火鸡（2.29%）中隐性存在。目前的研究结果突出了此类病毒在禽类、动物和人类H5N1流行病学中对禽类的严重影响。这也凸显了除疫苗接种外，还有其他原因导致H5N1隐性感染在埃及广泛传播。
