Mahmoud1 M A, El-Samawaty A M A, Yassin M A, Abd El-Aziz A R M
Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Genet Mol Res. 2016 Apr 28;15(2):gmr8081. doi: 10.4238/gmr.15028081.
Aspergillus flavus is one of the most abundant and widely distributed fungi on earth. A. flavus produces aflatoxins (AFs), which are toxic secondary metabolites. AFs have harmful effects on public health (humans and animals) and agricultural crops. Inter-simple sequence repeat (ISSR) markers were used to analyze the genetic diversity of 30 A. flavus isolates from five agricultural crops and air. Genetic similarity coefficients (GSC) ranged from 0.51 to 0.10 based on three ISSR markers for the isolates tested. A. flavus isolates grouped into 6, 5, and 3 clusters using the unweighted pair-group method with arithmetic average of three ISSR markers. This study suggests that ISSR biotechnology is a highly useful tool for characterizing genetic diversity of A. flavus isolated from different sources.
黄曲霉是地球上分布最广泛、数量最多的真菌之一。黄曲霉产生黄曲霉毒素（AFs），这是一种有毒的次生代谢产物。黄曲霉毒素对公众健康（人类和动物）以及农作物都有有害影响。利用简单重复序列区间（ISSR）标记分析了从五种农作物和空气中分离得到的30株黄曲霉的遗传多样性。基于所测试分离株的三个ISSR标记，遗传相似系数（GSC）在0.51至0.10之间。采用三个ISSR标记的非加权算术平均法对黄曲霉分离株进行聚类，可分为6个、5个和3个簇。本研究表明，ISSR生物技术是表征从不同来源分离得到的黄曲霉遗传多样性的一种非常有用的工具。
