Banerjee Aparajita, Rai Archana N, Penna Suprasanna, Variyar Prasad S
Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Food Chem. 2016 Oct 15;209:99-103. doi: 10.1016/j.foodchem.2016.04.022. Epub 2016 Apr 12.
Glucosinolates, found principally in the plant order Brassicales, are modulated by different post-harvest processing operations. Among these, ionizing radiation, a non-thermal process, has gained considerable interest for ensuring food security and safety. In gamma-irradiated cabbage, enhanced sinigrin, a major glucosinolate, has been reported. However, the molecular basis of such a radiation induced effect is not known. Herein, the effect of radiation processing on the expression of glucosinolate biosynthetic genes was investigated. RT-PCR based expression analysis of seven glucosinolate biosynthetic pathway genes (MYB28, CYP79F1, CYP83A1, SUR1, UGT74B1, SOT18 and TGG1) showed that CYP83A1, MYB28, UGT74B1, CYP79F1 and SUR1 were up-regulated in irradiated cabbage. The content of jasmonates, signalling molecules involved in glucosinolate induction was, however, unaffected in irradiated cabbage suggesting their non-involvement in glucosinolate induction during radiation processing. This is the first report on the effect of gamma irradiation on the expression of glucosinolate biosynthetic genes in vegetables.
硫代葡萄糖苷主要存在于十字花目植物中，会受到不同采后加工操作的影响。其中，电离辐射作为一种非热加工方式，在保障粮食安全和食品安全方面引起了广泛关注。据报道，在经伽马射线辐照的卷心菜中，主要硫代葡萄糖苷——黑芥子硫苷有所增加。然而，这种辐射诱导效应的分子基础尚不清楚。在此，我们研究了辐射加工对硫代葡萄糖苷生物合成基因表达的影响。基于RT-PCR对7个硫代葡萄糖苷生物合成途径基因（MYB28、CYP79F1、CYP83A1、SUR1、UGT74B1、SOT18和TGG1）的表达分析表明，在辐照后的卷心菜中，CYP83A1、MYB28、UGT74B1、CYP79F1和SUR1基因表达上调。然而，参与硫代葡萄糖苷诱导的信号分子茉莉酸类物质的含量在辐照后的卷心菜中并未受到影响，这表明它们在辐射加工过程中不参与硫代葡萄糖苷的诱导。这是关于伽马射线辐照对蔬菜中硫代葡萄糖苷生物合成基因表达影响的首次报道。
