Zang Yun-Xiang, Kim Jong-Hoon, Park Young-Doo, Kim Doo-Hwan, Hong Seung-Beom
Department of Molecular Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, Korea.
BMB Rep. 2008 Jun 30;41(6):472-8. doi: 10.5483/bmbrep.2008.41.6.472.
Three Arabidopsis cDNAs, MAM1, CYP79F1, and CYP83A1, required for aliphatic glucosinolate biosynthesis were introduced into Chinese cabbage by Agrobacterium tumefaciens-mediated transformation. The transgenic lines overexpressing MAM1 or CYP83A1 showed wild-type phenotypes. However, all the lines overexpressing CYP79F1 displayed phenotypes different from wild type with respect to the stem thickness as well as leaf width and shape. Glucosinolate contents of the transgenic plants were compared with those of wild type. In the MAM1 line M1-1, accumulation of aliphatic glucosinolates gluconapin and glucobrassicanapin significantly increased. In the CYP83A1 line A1-1, all the aliphatic glucosinolate levels were increased, and the levels of gluconapin and glucobrassicanapin were elevated by 4.5 and 2 fold, respectively. The three CYP79F1 transgenic lines exhibited dissimilar glucosinolate profiles. The F1-1 line accumulated higher levels of gluconapoleiferin, glucobrassicin, and 4-methoxy glucobrassicin. However, F1-2 and F1-3 lines demonstrated a decrease in the levels of gluconapin and glucobrassicanapin and an increased level of 4-hydroxy glucobrassicin.
通过根癌农杆菌介导的转化方法，将3个参与脂肪族硫代葡萄糖苷生物合成所需的拟南芥cDNA（MAM1、CYP79F1和CYP83A1）导入大白菜中。过表达MAM1或CYP83A1的转基因株系表现出野生型表型。然而，所有过表达CYP79F1的株系在茎粗以及叶宽和叶形方面均表现出与野生型不同的表型。对转基因植株的硫代葡萄糖苷含量与野生型进行了比较。在MAM1株系M1-1中，脂肪族硫代葡萄糖苷萝卜硫苷和芸苔葡糖硫苷的积累显著增加。在CYP83A1株系A1-1中，所有脂肪族硫代葡萄糖苷水平均升高，其中萝卜硫苷和芸苔葡糖硫苷的水平分别提高了4.5倍和2倍。3个CYP79F1转基因株系表现出不同的硫代葡萄糖苷谱。F1-1株系积累了较高水平的异硫氰酸烯丙酯、吲哚-3-甲醇和4-甲氧基吲哚-3-甲醇。然而，F1-2和F1-3株系中萝卜硫苷和芸苔葡糖硫苷水平降低，4-羟基吲哚-3-甲醇水平升高。
