Lan S, McCulloch E A, Till J E
J Natl Cancer Inst. 1978 Feb;60(2):265-9. doi: 10.1093/jnci/60.2.265.
Correlation analysis of numbers of colony-forming progenitor cells was used as an approach to the quantitation of human pluripotent stem cells. Marrow specimens were obtained from 24 patients with untreated acute myeloblastic leukemia, 22 patients under treatment, and 29 patients with no hematologic malignant disease. Three classes of progenitor cells were assayed: burst-forming units dependent on erythropoietin (BFU-E), colony-forming units dependent on erythropoietin (CFU-E), and granulopoietic progenitors (CFU-C). Significant positive correlations between numbers of BFU-E, CFU-E, and CFU-C were found in all 3 groups of patients. In contrast, no such positive correlations were seen between marrow blasts and any of the classes of colony-forming progenitors. These results were compatible with a shared relationship of the colony-forming progenitors to a pluripotent cell of origin and raised the possibility that the immediate progenitors of the blasts may not be any of the myelopoietic progenitor cells monitored in these studies.
集落形成祖细胞数量的相关性分析被用作定量人类多能干细胞的一种方法。从24例未经治疗的急性髓细胞性白血病患者、22例正在接受治疗的患者以及29例无血液系统恶性疾病的患者身上获取骨髓标本。检测了三类祖细胞：依赖促红细胞生成素的爆式集落形成单位（BFU-E）、依赖促红细胞生成素的集落形成单位（CFU-E）和粒细胞祖细胞（CFU-C）。在所有3组患者中均发现BFU-E、CFU-E和CFU-C数量之间存在显著正相关。相比之下，在骨髓原始细胞与任何一类集落形成祖细胞之间未观察到这种正相关。这些结果与集落形成祖细胞与多能起源细胞的共同关系相符，并提出这些研究中监测的髓系祖细胞可能不是原始细胞的直接祖细胞的可能性。
