Nustede R, Köhler H, Pfannkuche A, Fölsch U R, Schafmayer A
Department of General Surgery, University of Göttingen, F.R.G.
Pancreas. 1989;4(1):114-9. doi: 10.1097/00006676-198902000-00018.
Stimulation of the exocrine pancreas is one of the biological effects attributed to the tridecapeptide neurotensin (NT). Only the C-terminal 8-13 fragment with its extremely short plasma half-life in the peripheral circulation was considered to be necessary for biological activity. The N-terminal fragments that are detectable in peripheral blood in much greater concentrations were considered to be of no importance. In order to elucidate the biological relevance of these N-terminal fragments, five dogs were infused with neurotensin at a rate (50 pmol/kg/h) that had been determined to give physiological plasma concentrations. Pancreatic secretions were collected from previously implanted modified Herrera pouches. The effects of the N-terminal fragments NT 1-8 and NT 1-11 on the exocrine pancreas secretion were tested in the same way. It was possible for the first time to demonstrate a biological effect of the N-terminal fragments found in the postprandial circulation in vivo. The plasma concentrations determined by radioimmunoassay remained within the physiological range (less than 40 pg/ml). These N-terminal neurotensin fragments may therefore participate in the hormonal regulation of the exocrine pancreas secretion.
外分泌胰腺的刺激是十三肽神经降压素（NT）的生物学效应之一。只有C末端8 - 13片段及其在外周循环中极短的血浆半衰期被认为对生物活性是必需的。在外周血中以高得多的浓度可检测到的N末端片段被认为不重要。为了阐明这些N末端片段的生物学相关性，以已确定能产生生理血浆浓度的速率（50 pmol/kg/h）给5只狗输注神经降压素。从先前植入的改良埃雷拉袋收集胰腺分泌物。以同样的方式测试N末端片段NT 1 - 8和NT 1 - 11对外分泌胰腺分泌的影响。首次有可能证明在体内餐后循环中发现的N末端片段的生物学效应。通过放射免疫测定法测定的血浆浓度保持在生理范围内（小于40 pg/ml）。因此，这些N末端神经降压素片段可能参与外分泌胰腺分泌的激素调节。
