Nustede R, Schmidt W E, Köhler H, Fölsch U R, Schafmayer A
Department of Surgery, Georg-August-University Göttingen, Germany.
Regul Pept. 1993 Mar 5;44(1):25-32. doi: 10.1016/0167-0115(93)90127-t.
A potential physiological function of the regulatory gastrointestinal peptide neurotensin (NT) is the stimulation of exocrine pancreatic secretion. We investigated therefore whether immunoneutralization of the postprandially circulating peptide, intravenous application of either atropine or the highly specific CCK receptor antagonist, L-364,718, could influence neurotensin-mediated pancreatic secretion in dogs. The use of CCK receptor antagonist (0.1 mg kg-1 intraduodenally) inhibited postprandial and NT-mediated exocrine pancreatic secretion. The integrated postprandial protein secretion fell from 31 +/- 1.6 to 23 +/- 2.1 g 180 min-1 while the corresponding values in response to i.v.-NT (postprandial neurotensin concentration course was imitated by the infusion of 50 pmol kg h-1 NT) fell from 22 +/- 1.9 to 7.5 +/- 0.8 g 180 min-1. Immunoneutralization of postprandial NT led to a simultaneous significant reduction in postprandial pancreatic secretion (integrated protein release 31 +/- 1.6 and 15.5 +/- 1.4 g 180 min-1 respectively). The i.v. application of atropine lowered NT-mediated pancreatic secretion (e.g., protein output) from 22 +/- 1.9 to 7.1 +/- 7.1 +/- 0.9 g 180 min-1. We conclude that NT plays an important role in the endocrine regulation of exocrine pancreatic secretion. This influence could be mediated by a CCK-dependent cholinergic mechanism.
调节性胃肠肽神经降压素（NT）的一种潜在生理功能是刺激胰腺外分泌。因此，我们研究了对餐后循环肽进行免疫中和、静脉注射阿托品或高度特异性的CCK受体拮抗剂L-364,718是否会影响犬体内神经降压素介导的胰腺分泌。使用CCK受体拮抗剂（0.1 mg kg-1十二指肠内给药）可抑制餐后和神经降压素介导的胰腺外分泌。餐后蛋白质分泌总量从31±1.6降至23±2.1 g 180分钟-1，而静脉注射神经降压素（通过以50 pmol kg h-1的速度输注神经降压素模拟餐后神经降压素浓度变化过程）后的相应值从22±1.9降至7.5±0.8 g 180分钟-1。对餐后神经降压素进行免疫中和导致餐后胰腺分泌同时显著减少（蛋白质释放总量分别为31±1.6和15.5±1.4 g 180分钟-1）。静脉注射阿托品可降低神经降压素介导的胰腺分泌（如蛋白质输出量），从22±1.9降至7.1±0.9 g 180分钟-1。我们得出结论，神经降压素在胰腺外分泌的内分泌调节中起重要作用。这种影响可能由CCK依赖性胆碱能机制介导。
