Beaulieu Aimee M, Sun Joseph C
Department of Microbiology, Biochemistry and Moleculor Genetics, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, New York, NJ, 07103, USA.
Immunology Department, Memorial Sloan-Kettering Cancer Center, 408 East 69th Street, ZRC-1402, New York, NY, 10065, USA.
Methods Mol Biol. 2016;1441:1-12. doi: 10.1007/978-1-4939-3684-7_1.
In C57BL/6 mice, NK cells expressing the activating receptor Ly49H proliferate robustly in response to mouse cytomegalovirus (MCMV) infection. The expansion of Ly49H(+) NK cells peaks at approximately 1 week post-infection, and is then followed by a distinct contraction phase that ultimately leaves a small but long-lived pool of MCMV-experienced Ly49H(+) NK cells that are capable of mediating enhanced memory-like responses during subsequent encounters with MCMV. Here we describe an adoptive transfer model in which the expansion, contraction, and memory cell persistence of transferred Ly49H(+) NK cells are tracked in congenic C57BL/6 hosts following MCMV infection.
在C57BL/6小鼠中，表达激活受体Ly49H的自然杀伤（NK）细胞在受到小鼠巨细胞病毒（MCMV）感染时会强烈增殖。Ly49H(+) NK细胞的扩增在感染后约1周达到峰值，随后是一个明显的收缩期，最终留下一小群长寿的、经历过MCMV的Ly49H(+) NK细胞，这些细胞在随后再次接触MCMV时能够介导增强的类似记忆的反应。在此，我们描述了一种过继转移模型，在该模型中，追踪了MCMV感染后同基因C57BL/6宿主中转移的Ly49H(+) NK细胞的扩增、收缩和记忆细胞持久性。
