巨细胞病毒感染：小鼠模型
Cytomegalovirus Infection: Mouse Model.
作者信息
Brizić Ilija, Lisnić Berislav, Brune Wolfram, Hengel Hartmut, Jonjić Stipan
机构信息
Department of Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
出版信息
Curr Protoc Immunol. 2018 Aug;122(1):e51. doi: 10.1002/cpim.51. Epub 2018 Jul 25.
Abstract
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay. © 2018 by John Wiley & Sons, Inc.
摘要
本单元描述了用鼠巨细胞病毒（MCMV）感染新生小鼠和成年小鼠的程序。其中包括在细胞培养物中繁殖MCMV以及从感染小鼠的唾液腺制备毒性更强的MCMV形式的方法。提供了一种噬斑测定法，用于确定感染组织或病毒原液的MCMV滴度。此外，还描述了一种制备用于繁殖MCMV和噬斑测定的小鼠胚胎成纤维细胞的方法。© 2018约翰威立父子公司版权所有。
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