Advanced Functional Materials Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh 202002, India.
Chemistry Department, Faculty of Science, King Abdulaziz University, P. O. Box 80203, Jeddah 21589, Saudi Arabia; Centre of Excellence for Advanced Materials Research (CEAMR), King Abdulaziz University, P. O. Box 80203, Jeddah 21589, Saudi Arabia.
Int J Biol Macromol. 2018 Jan;106:755-762. doi: 10.1016/j.ijbiomac.2017.08.074. Epub 2017 Aug 18.
This study is based on the construction of an enzymatic bioanode adopting the exclusively reported layer-by-layer (LBL) assembly of Ppy-Ag-GO/ferritin (Frt)/glucose oxidase (GOx). The glassy carbon (GC) electrode was immobilised with the conducting polypyrrole (Ppy)-silver nanoparticles (Ag)-graphene oxide (GO) based biocomposite as electron transfer elevator, horse spleen ferritin (Frt) protein as electron transfer mediator and glucose oxidase (GOx) enzyme in layer by layer configuration. The fabricated bioanode exhibited good electrochemical performance with a maximum current response of 5.7mAcm accompanied with biocompatibility and environmental stability because of the synergistic effect between outstanding properties of PPy, silver and GO, thereby, showing superior catalytic efficiency for the oxidation of glucose.
本研究基于酶生物阳极的构建，采用独特报道的层层（LBL）组装法将 Ppy-Ag-GO/铁蛋白（Frt）/葡萄糖氧化酶（GOx）组装在一起。玻碳电极通过基于导电聚吡咯（Ppy）-银纳米粒子（Ag）-氧化石墨烯（GO）的生物复合材料固定化，该复合材料作为电子转移提升剂，马脾铁蛋白（Frt）蛋白作为电子转移介体，葡萄糖氧化酶（GOx）酶以层层结构形式存在。由于 PPy、银和 GO 的卓越性能之间的协同作用，所构建的生物阳极具有良好的电化学性能，最大电流响应为 5.7mAcm，同时具有生物相容性和环境稳定性，因此对葡萄糖的氧化表现出优异的催化效率。
