Dale Erica A, Fields Daryl P, Devinney Michael J, Mitchell Gordon S
Department of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, United States.
Department of Comparative Biosciences, University of Wisconsin, Madison, WI 53706, United States; Department of Physical Therapy and McKnight Brain Institute, University of Florida, Gainesville, FL 32610, United States.
Exp Neurol. 2017 Jan;287(Pt 2):130-136. doi: 10.1016/j.expneurol.2016.05.012. Epub 2016 May 13.
Phrenic long-term facilitation (pLTF) is a form of hypoxia-induced spinal respiratory motor plasticity that requires new synthesis of brain derived neurotrophic factor (BDNF) and activation of its high-affinity receptor, tropomyosin receptor kinase B (TrkB). Since the cellular location of relevant TrkB receptors is not known, we utilized intrapleural siRNA injections to selectively knock down TrkB receptor protein within phrenic motor neurons. TrkB receptors within phrenic motor neurons are necessary for BDNF-dependent acute intermittent hypoxia-induced pLTF, demonstrating that phrenic motor neurons are a critical site of respiratory motor plasticity.
膈神经长期易化（pLTF）是一种低氧诱导的脊髓呼吸运动可塑性形式，它需要脑源性神经营养因子（BDNF）的新合成及其高亲和力受体原肌球蛋白受体激酶B（TrkB）的激活。由于相关TrkB受体的细胞定位尚不清楚，我们利用胸膜腔内注射小干扰RNA（siRNA）来选择性敲除膈运动神经元内的TrkB受体蛋白。膈运动神经元内的TrkB受体对于BDNF依赖的急性间歇性低氧诱导的pLTF是必需的，这表明膈运动神经元是呼吸运动可塑性的关键部位。
