Maor G, Hochberg Z, Silbermann M
Laboratory for Musculoskeletal Research, Rappaport Institute for Research, Faculty of Medicine, Haifa, Israel.
Acta Endocrinol (Copenh). 1989 Apr;120(4):526-32. doi: 10.1530/acta.0.1200526.
This study used an organ culture system of neonatal condylar cartilage to study the in vitro effects of recombinant human growth hormone on the growth of cartilage and its inherent cell populations: progenitor cells, chondroblasts and early hypertrophic chondrocytes. Growth hormone at a dose of 2.5 nmol/l enhanced the overall growth of cartilage explant and stimulated the differentiation of its cells. Hence, growth hormone-treated explants revealed a substantial increase in the number of chondroblasts and young hypertrophic chondrocytes. Along with its effects upon cartilage the hormone also stimulated new bone formation adjacent to mineralized hypertrophic chondrocytes. These results provide support to the notion that growth hormone stimulates cartilage growth which in turn is followed by endochondral ossification. In spite of its in vitro effect it is not as yet clear whether the effect of growth hormone is indeed a direct one or is mediated via the local production of IGF-I.
本研究采用新生儿髁突软骨器官培养系统，以研究重组人生长激素对软骨及其固有细胞群体（祖细胞、成软骨细胞和早期肥大软骨细胞）生长的体外影响。2.5 nmol/l剂量的生长激素可增强软骨外植体的整体生长，并刺激其细胞分化。因此，经生长激素处理的外植体显示成软骨细胞和年轻肥大软骨细胞的数量大幅增加。该激素在影响软骨的同时，还刺激了矿化肥大软骨细胞附近新骨的形成。这些结果支持了生长激素刺激软骨生长进而引发软骨内骨化的观点。尽管生长激素在体外有作用，但目前尚不清楚其作用是否确实是直接的，还是通过局部产生胰岛素样生长因子-I介导的。
