Żywicka Anna, Peitler Dorota, Rakoczy Rafał, Junka Adam Feliks, Fijałkowski Karol
Department of Immunology, Microbiology and Physiological Chemistry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311, Szczecin, Poland.
Institute of Chemical Engineering and Environmental Protection Processes, West Pomeranian University of Technology, Szczecin, Piastów 42, 71-065, Szczecin, Poland.
Appl Biochem Biotechnol. 2016 Oct;180(4):805-816. doi: 10.1007/s12010-016-2134-4. Epub 2016 May 17.
The present study aimed to explore and describe the properties of bacterial cellulose (BC) membranes obtained from three different strains of Gluconacetobacter xylinus for 72, 120, and 168 h, used as a carrier support for the immobilization of Saccharomyces cerevisiae. The experiments also included the analysis of glucose consumption and alcohol production during the fermentation process displayed by yeasts immobilized on the BC surface. The results of the present study demonstrate that the number of immobilized yeast cells is dependent on the type of cellulose-synthesizing strain, cellulose form, and duration of its synthesis. The BC in the form of wet membranes obtained after 3 days of synthesis displayed the most favorable properties as a carrier for yeast immobilization. The immobilization of yeast cells on BC, regardless of its form, increased the amount of the produced alcohol as compared to free cells. The yeast cells immobilized in BC were able to multiply on its surface during the fermentation process.
本研究旨在探索和描述从三种不同木醋杆菌菌株中获得的细菌纤维素(BC)膜在72、120和168小时时的特性,这些BC膜用作固定酿酒酵母的载体支持物。实验还包括分析固定在BC表面的酵母在发酵过程中葡萄糖消耗和酒精产生情况。本研究结果表明,固定化酵母细胞的数量取决于纤维素合成菌株的类型、纤维素形式及其合成持续时间。合成3天后获得的湿膜形式的BC作为酵母固定化载体表现出最有利的特性。与游离细胞相比,无论BC形式如何,酵母细胞固定在BC上都会增加酒精产量。固定在BC中的酵母细胞在发酵过程中能够在其表面繁殖。