Huang C, Yang X-Y, Xiong L, Guo H-J, Luo J, Wang B, Zhang H-R, Lin X-Q, Chen X-D
Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou, China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, China.
Lett Appl Microbiol. 2015 May;60(5):491-6. doi: 10.1111/lam.12396. Epub 2015 Feb 12.
To reduce the cost of bacterial cellulose (BC) production, the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater with high COD value (18 050 mg l(-1) ) for BC production by Gluconacetobacter xylinus was evaluated. After 7 days of fermentation, the highest BC yield (1·34 g l(-1) ) was obtained. The carbon sources including sugars (glucose and xylose), organic acids (acetic acid and butyric acid) and alcohol compounds (ethanol and butanol) were utilized by G. xylinus simultaneously during fermentation. Although the COD decrease ratio (about 14·7%) was low, the highest BC yield on COD consumption (56·2%, g g(-1) ) was relatively high and the remaining wastewater could be used for further BC fermentation. Besides, the environment of ABE fermentation wastewater showed small influence on the BC structure by comparison with the BC products obtained in traditional HS medium using field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Overall, ABE fermentation wastewater is one promising substrate for BC production.
The possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose (BC) production by Gluconacetobacter xylinus was evaluated in this study. This is the first time that ABE fermentation wastewater was used as substrate for BC fermentation. The results provide detail information of metabolism of G. xylinus in ABE fermentation wastewater and the influence of wastewater environment on the structure of BC samples. Overall, this bioconversion could reduce the cost of BC production greatly.
为降低细菌纤维素（BC）的生产成本，评估了使用化学需氧量（COD）值较高（18050 mg l⁻¹）的丙酮 - 丁醇 - 乙醇（ABE）发酵废水来生产木醋杆菌属木糖杆菌BC的可能性。发酵7天后，获得了最高的BC产量（1.34 g l⁻¹）。在发酵过程中，木醋杆菌属木糖杆菌同时利用了包括糖类（葡萄糖和木糖）、有机酸（乙酸和丁酸）以及醇类化合物（乙醇和丁醇）在内的碳源。尽管COD降低率（约14.7%）较低，但基于COD消耗的最高BC产量（56.2%，g g⁻¹）相对较高，且剩余废水可用于进一步的BC发酵。此外，通过场发射扫描电子显微镜（FE - SEM）、傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）比较发现，与在传统HS培养基中获得的BC产品相比，ABE发酵废水环境对BC结构的影响较小。总体而言，ABE发酵废水是一种有前景的BC生产底物。
本研究评估了使用丙酮 - 丁醇 - 乙醇（ABE）发酵废水来生产木醋杆菌属木糖杆菌细菌纤维素（BC）的可能性。这是首次将ABE发酵废水用作BC发酵的底物。研究结果提供了木醋杆菌属木糖杆菌在ABE发酵废水中的代谢详细信息以及废水环境对BC样品结构产生的影响。总体而言，这种生物转化可以大幅降低BC的生产成本。
