Bellasio Martina, Peymann Armin, Steiger Matthias G, Valli Minoska, Sipiczki Matthias, Sauer Michael, Graf Alexandra B, Marx Hans, Mattanovich Diethard
Department of Biotechnology, BOKU-VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
Austrian Centre of Industrial Biotechnology (ACIB), University of Applied Sciences FH Campus Wien, 1190 Vienna, Austria School of Bioengineering, University of Applied Sciences FH Campus Wien, 1190 Vienna, Austria.
FEMS Yeast Res. 2016 Jun;16(4). doi: 10.1093/femsyr/fow037. Epub 2016 Apr 17.
Efficient conversion of hexoses and pentoses into value-added chemicals represents one core step for establishing economically feasible biorefineries from lignocellulosic material. While extensive research efforts have recently provided advances in the overall process performance, the quest for new microbial cell factories and novel enzymes sources is still open. As demonstrated recently the yeast Sugiyamaella lignohabitans (formerly Candida lignohabitans) represents a promising microbial cell factory for the production of organic acids from lignocellulosic hydrolysates. We report here the de novo genome assembly of S. lignohabitans using the Single Molecule Real-Time platform, with gene prediction refined by using RNA-seq. The sequencing revealed a 15.98 Mb genome, subdivided into four chromosomes. By phylogenetic analysis, Blastobotrys (Arxula) adeninivorans and Yarrowia lipolytica were found to be close relatives of S. lignohabitans Differential gene expression was evaluated in typical growth conditions on glucose and xylose and allowed a first insight into the transcriptional response of S. lignohabitans to different carbon sources and different oxygenation conditions. Novel sequences for enzymes and transporters involved in the central carbon metabolism, and therefore of potential biotechnological interest, were identified. These data open the way for a better understanding of the metabolism of S. lignohabitans and provide resources for further metabolic engineering.
将己糖和戊糖高效转化为高附加值化学品是利用木质纤维素材料建立经济可行的生物精炼厂的核心步骤之一。尽管最近大量的研究工作在整体工艺性能方面取得了进展，但对新型微生物细胞工厂和新型酶源的探索仍在继续。最近的研究表明，木质栖居糖酵母（以前称为木质栖居假丝酵母）是一种有前景的微生物细胞工厂，可用于从木质纤维素水解产物中生产有机酸。我们在此报告了使用单分子实时平台对木质栖居糖酵母进行的从头基因组组装，并通过RNA测序对基因预测进行了优化。测序结果显示其基因组大小为15.98 Mb，分为四条染色体。通过系统发育分析，发现嗜腺嘌呤芽殖酵母和解脂耶氏酵母是木质栖居糖酵母的近亲。在葡萄糖和木糖的典型生长条件下评估了差异基因表达，从而初步了解了木质栖居糖酵母对不同碳源和不同氧化条件的转录反应。鉴定出了参与中心碳代谢的酶和转运蛋白的新序列，因此具有潜在的生物技术意义。这些数据为更好地理解木质栖居糖酵母的代谢开辟了道路，并为进一步的代谢工程提供了资源。
