He Qina, Sun Yulong, Zong Lin, Tong Jian, Cao Yi
School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, China.
Biomed Res Int. 2016;2016:4918691. doi: 10.1155/2016/4918691. Epub 2016 Apr 14.
Background. Several investigators have reported increased levels of poly(ADP-ribose) polymerase-1 (PARP-1), a nuclear enzyme which plays an important role in the repair of damaged DNA, in cells exposed to extremely low dose ionizing radiation which does not cause measurable DNA damage. Objective. To examine whether exposure of the cells to nonionizing radiofrequency fields (RF) is capable of increasing messenger RNA of PARP-1 and its protein levels in mouse bone marrow stromal cells (BMSCs). Methods. BMSCs were exposed to 900 MHz RF at 120 μW/cm(2) power intensity for 3 hours/day for 5 days. PARP-1 mRNA and its protein levels were examined at 0, 0.5, 1, 2, 4, 6, 8, and 10 hours after exposure using RT-PCR and Western blot analyses. Sham-exposed (SH) cells and those exposed to ionizing radiation were used as unexposed and positive control cells. Results. BMSCs exposed to RF showed significantly increased expression of PARP-1 mRNA and its protein levels after exposure to RF while such changes were not observed in SH-exposed cells. Conclusion. Nonionizing RF exposure is capable of inducing PARP-1.
背景。几位研究者报告称，在暴露于不会造成可测量DNA损伤的极低剂量电离辐射的细胞中，聚（ADP - 核糖）聚合酶 - 1（PARP - 1）水平升高，PARP - 1是一种在受损DNA修复中起重要作用的核酶。目的。研究细胞暴露于非电离射频场（RF）是否能够增加小鼠骨髓基质细胞（BMSC）中PARP - 1的信使核糖核酸及其蛋白质水平。方法。将BMSC暴露于功率强度为120 μW/cm²的900 MHz RF下，每天3小时，共5天。在暴露后0、0.5、1、2、4、6、8和10小时，使用逆转录 - 聚合酶链反应（RT - PCR）和蛋白质印迹分析检测PARP - 1信使核糖核酸及其蛋白质水平。假暴露（SH）细胞和暴露于电离辐射的细胞分别用作未暴露和阳性对照细胞。结果。暴露于RF的BMSC在暴露于RF后显示PARP - 1信使核糖核酸及其蛋白质水平显著增加，而在SH暴露细胞中未观察到此类变化。结论。非电离RF暴露能够诱导PARP - 1。
