Quero Sara, García-Núñez Marian, Párraga-Niño Noemí, Barrabeig Irene, Pedro-Botet Maria L, de Simon Mercè, Sopena Nieves, Sabrià Miquel
Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916.
Universitat Autònoma de Barcelona, Cerdanyola, Spain E-08193.
Future Microbiol. 2016 Jun;11:757-65. doi: 10.2217/fmb-2015-0030. Epub 2016 May 18.
To compare the discriminatory power of pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) in Legionella outbreaks for determining the infection source.
MATERIALS & METHODS: Twenty-five investigations of Legionnaires' disease were analyzed by PFGE, SBT and Dresden monoclonal antibody.
The results suggested that monoclonal antibody could reduce the number of Legionella isolates to be characterized by molecular methods. The epidemiological concordance PFGE-SBT was 100%, while the molecular concordance was 64%. Adjusted Wallace index (AW) showed that PFGE has better discriminatory power than SBT (AWSBT→PFGE = 0.767; AWPFGE→SBT = 1). The discrepancies appeared mostly in sequence type (ST) 1, a worldwide distributed ST for which PFGE discriminated different profiles.
SBT discriminatory power was not sufficient verifying the infection source, especially in worldwide distributed STs, which were classified into different PFGE patterns.
比较脉冲场凝胶电泳（PFGE）和基于序列的分型方法（SBT）在军团菌暴发中确定感染源的鉴别能力。
采用PFGE、SBT和德累斯顿单克隆抗体对25例军团病调查进行分析。
结果表明，单克隆抗体可减少需用分子方法鉴定的军团菌分离株数量。PFGE与SBT的流行病学一致性为100%，而分子一致性为64%。调整后的华莱士指数（AW）显示，PFGE的鉴别能力优于SBT（AW SBT→PFGE = 0.767；AW PFGE→SBT = 1）。差异主要出现在序列型（ST）1中，这是一种全球分布的序列型，PFGE可区分不同的图谱。
SBT的鉴别能力不足以验证感染源，尤其是在全球分布的序列型中，这些序列型被分为不同的PFGE模式。
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