Grosskopf R K, Grosskopf H M, Boito J P, Bottari N B, Machado G, Biazus A H, Schetinger M R C, Morsch V M, Tonin A A, Paiano D, Balzan A, da Silva A S
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil.
Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, Brazil.
J Anim Physiol Anim Nutr (Berl). 2017 Apr;101(2):243-250. doi: 10.1111/jpn.12526. Epub 2016 May 18.
This study aimed to evaluate the effect of milk feeding using milk replacer (MR) or natural ewe's milk (NEM) for Lacaune lambs on performance, protein and lipid metabolism and oxidative/antioxidant status. These parameters were evaluated during the period of feeding adaptation, which corresponded to 12 days after ewe's separation. Fifteen lambs were selected and divided into two groups: Group A (n = 7) with lambs receiving natural milk; Group B (n = 8) with lambs receiving a milk replacer. Liquid food supply, for both groups, was set according to their body weight, given in two daily periods. Feed intake was also obtained by individual body weight and adjusted according leftovers. Blood samples were collected on three different days (0, 6 and 12) to perform the serum analysis of total protein, albumin, cholesterol, triglycerides, alanine aminotransferase (ALT) and urea. Additionally, the oxidative profile was analysed in sera samples through the assessment of TBARS (reactive substances thiobarbituric acid) and ROS (reactive oxygen species) which aims to identify lipid peroxidation and free radical levels, respectively, as well as the antioxidant profile evaluating glutathione S-transferase (GST). There was no statistical difference (p > 0.05) between groups on levels of total protein, albumin and globulin. However, cholesterol (day 6), triglycerides (days 6 and 12), urea (day 6) and ALT (day 12) levels differed (p < 0.05) between groups. Values of total protein, globulin, ALT and TBARS differed (p < 0.05) along the time. The average weight gain and body weight differed (p < 0.05) only on day 12, showing that weight gain was higher for lambs fed with NEM. The performance of lambs fed with MR was impaired. Biochemical parameters had a slight variation between groups and along the time, as well as oxidative/antioxidant status did not change in the different liquid diets.
本研究旨在评估使用代乳品（MR）或天然羊奶（NEM）喂养拉库内羔羊对其生长性能、蛋白质和脂质代谢以及氧化/抗氧化状态的影响。在母羊与羔羊分离后的12天适应期内对这些参数进行评估。挑选了15只羔羊并分为两组：A组（n = 7），羔羊接受天然奶；B组（n = 8），羔羊接受代乳品。两组的液体食物供应量均根据其体重设定，每天分两次投喂。采食量也根据个体体重获取，并根据剩余量进行调整。在三个不同日期（0、6和12天）采集血样，以进行血清总蛋白、白蛋白、胆固醇、甘油三酯、丙氨酸转氨酶（ALT）和尿素的分析。此外，通过评估硫代巴比妥酸反应物质（TBARS）和活性氧（ROS）来分析血清样本中的氧化状态，目的分别是确定脂质过氧化和自由基水平，同时评估谷胱甘肽S-转移酶（GST）来评估抗氧化状态。两组之间总蛋白、白蛋白和球蛋白水平无统计学差异（p>0.05）。然而，两组之间胆固醇（第6天）、甘油三酯（第6天和第12天）、尿素（第6天）和ALT（第12天）水平存在差异（p<0.05）。总蛋白、球蛋白、ALT和TBARS的值随时间变化（p<0.05）。平均体重增加和体重仅在第12天存在差异（p<0.05），表明用NEM喂养的羔羊体重增加更高。用MR喂养的羔羊生长性能受损。不同组之间生化参数以及氧化/抗氧化状态在不同液体日粮之间略有变化。
