Shors S T, Winston V
Department of Biological Sciences, Idaho State University, Pocatello 83209.
Am J Vet Res. 1989 Feb;50(2):232-4.
Neutralizing antibodies specific for infectious hematopoietic necrosis virus (IHNV) were isolated from eggs of spawning Steelhead trout (Salmo gairdneri), using sodium sulfate precipitation. The isolated material was used in place of the primary antibody (rabbit anti-IHNV) in a protein immunoblotting assay to detect IHNV proteins specifically. The egg component that bound specifically to IHNV proteins was determined to be trout antibody by using antiserum toward trout immune globulins as the second antibody conjugate in the protein immunoblotting assay. The antibody recovered from eggs neutralized IHNV infectivity in cell culture. An average of 87.5% decrease in infectivity was observed.
使用硫酸钠沉淀法，从正在产卵的虹鳟（Salmo gairdneri）鱼卵中分离出对传染性造血器官坏死病毒（IHNV）具有特异性的中和抗体。在蛋白质免疫印迹分析中，使用分离出的物质替代一抗（兔抗IHNV），以特异性检测IHNV蛋白。在蛋白质免疫印迹分析中，通过使用针对鳟鱼免疫球蛋白的抗血清作为第二抗体偶联物，确定与IHNV蛋白特异性结合的鱼卵成分是鳟鱼抗体。从鱼卵中回收的抗体在细胞培养中中和了IHNV的感染性。观察到感染性平均降低了87.5%。
