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[从用聚丙烯酰胺凝胶电泳中分离出的最慢血清组分免疫的兔获得抗虹鳟鱼(Salmo gairdneri Rich.)免疫球蛋白特异性血清]

[Obtaining an anti-trout (Salmo gairdneri Rich.) immunoglobulin specific serum from rabbits immunized with the slowest serum fraction isolated in polyacrylamide gel electrophoresis].

作者信息

Dorson M, Perrier H, Perrier C

出版信息

C R Seances Acad Sci D. 1980 Jan 14;290(2):101-4.

PMID:6771043
Abstract

Rabbits were immunized with the slowest fraction obtained by polyacrylamide gel electrophoresis of normal Trout serum previously cleared of lipoproteins. The antiserum thus obtained reacts specifically with Trout immunoglobulin. This was verified by inhibiting the neutralization of Infectious Pancreatic Necrosis Virus by Trout antibodies, and by comparing it in immunoelectrophoresis with a rabbit antiserum previously obtained by immunization with Trout antibodies-Dinitrophenol-Haemocyanin immune complexes.

摘要

用正常鳟鱼血清经聚丙烯酰胺凝胶电泳分离得到的最慢组分对兔子进行免疫,该血清此前已去除脂蛋白。由此获得的抗血清与鳟鱼免疫球蛋白发生特异性反应。这通过抑制鳟鱼抗体对传染性胰腺坏死病毒的中和作用得到证实,并通过免疫电泳将其与先前用鳟鱼抗体 - 二硝基苯酚 - 血蓝蛋白免疫复合物免疫获得的兔抗血清进行比较得以验证。

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