Condro Michael C, Matynia Anna, Foster Nicholas N, Ago Yukio, Rajbhandari Abha K, Van Christina, Jayaram Bhavaani, Parikh Sachin, Diep Anna L, Nguyen Eileen, May Victor, Dong Hong-Wei, Waschek James A
Department of Psychiatry, University of California, Los Angeles, USA 90095.
Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, USA 90095.
J Comp Neurol. 2016 Dec 15;524(18):3827-3848. doi: 10.1002/cne.24035. Epub 2016 Jun 3.
Pituitary adenylate cyclase-activating polypeptide (PACAP, gene name Adcyap1) regulates a wide variety of neurological and physiological functions, including metabolism and cognition, and plays roles in of multiple forms of stress. Because of its preferential expression in nerve fibers, it has often been difficult to trace and identify the endogenous sources of the peptide in specific populations of neurons. Here, we introduce a transgenic mouse line that harbors in its genome a bacterial artificial chromosome containing an enhanced green fluorescent protein (EGFP) expression cassette inserted upstream of the PACAP ATG translation initiation codon. Analysis of expression in brain sections of these mice using a GFP antibody reveals EGFP expression in distinct neuronal perikarya and dendritic arbors in several major brain regions previously reported to express PACAP from using a variety of approaches, including radioimmunoassay, in situ hybridization, and immunohistochemistry with and without colchicine. EGFP expression in neuronal perikarya was modulated in a manner similar to PACAP gene expression in motor neurons after peripheral axotomy in the ipsilateral facial motor nucleus in the brainstem, providing an example in which the transgene undergoes proper regulation in vivo. These mice and the high-resolution map obtained are expected to be useful in understanding the anatomical patterns of PACAP expression and its plasticity in the mouse. J. Comp. Neurol. 524:3827-3848, 2016. © 2016 Wiley Periodicals, Inc.
垂体腺苷酸环化酶激活多肽（PACAP，基因名Adcyap1）调节多种神经和生理功能，包括新陈代谢和认知，并在多种应激形式中发挥作用。由于其在神经纤维中的优先表达，通常很难追踪和确定特定神经元群体中该肽的内源性来源。在此，我们引入一种转基因小鼠品系，其基因组中含有一个细菌人工染色体，该染色体在PACAP的ATG翻译起始密码子上游插入了一个增强型绿色荧光蛋白（EGFP）表达盒。使用GFP抗体对这些小鼠脑切片中的表达进行分析，发现在先前使用包括放射免疫分析、原位杂交以及有无秋水仙碱的免疫组织化学等多种方法报道表达PACAP的几个主要脑区中，不同的神经元胞体和树突分支中有EGFP表达。在脑干同侧面神经运动核外周轴突切断后，神经元胞体中的EGFP表达以类似于运动神经元中PACAP基因表达的方式受到调节，这提供了一个转基因在体内受到适当调节的例子。这些小鼠以及获得的高分辨率图谱有望有助于理解PACAP在小鼠中的表达解剖模式及其可塑性。《比较神经学杂志》524:3827 - 3848，2016年。© 2016威利期刊公司。
